Analgesic effect of exercise is long been debated and controversial topic as it may result in hyperalgesia or hypoalgesia. The aim of the study is to compare the effect of exercise on cold induced acute pain in male and female. The subjects were asked to immerse his/her dominant hand in ice cold water (20 to 40C) and pain threshold (start of feeling pain) and pain tolerance time (total time up to which pain can be tolerated) were recorded. Blood pressure, heart rate and respiratory rate were recorded during and after cold pressor pain test and exercise to study cardiovascular effects. To minimize the analgesic effects of female sex hormones, experiments on females were done in mid-luteal phase (21 to 24 days of menstrual cycle). For exercise, bicycle ergometer was used. Initial load for exercise was 25 watts, which increases with 25 watts at the interval of every 2 minutes and exercise was continued until the heart rate reached the 60 to 75 % of the maximal heart rate for that subject. There was significant increase in all pain parameters just after exercise (Pain threshold from 14.25 ±10.2 sec to 20.83±13sec after exercise, p< 0.001; pain tolerance from 39.5 ±25 sec to 54.67±31 sec, p<0.001). Exercise had much effect on pain tolerance than pain threshold (from 26.2±20 sec to 36.2±23.5 sec, p<0.01). The effect of exercise on pain perception was significant in both male and female, the analgesic effect being more enhanced in female than male. Exercise-induced hypoalgesia: potential mechanisms in animal models of neuropathic pain. Schmitt A, Wallat D, Stangier C, Martin JA, Schlesinger-Irsch U, Boecker H.et al., Eur J Pain. 2019 Nov. doi: 10.1002/ejp.1508. [Epub ahead of print] Exercise-induced hypoalgesia: A meta-analysis of exercise dosing for the treatment of chronic pain. Polaski AM, Phelps AL, Kostek MC, Szucs KA, Kolber BJ. PLoS One. 2019 Jan 9;14(1): e0210418 The analgesic effect of music on cold pressor pain responses: The influence of anxiety and attitude toward pain. Choi S, Park SG, Lee HH. PLoS One. 2018; 6: 13(8). Exercise-induced hypoalgesia: potential mechanisms in animal models of neuropathic pain. Kami K, Tajima F, Senba E. Anat Sci Int. 2017; 92(1):79-90. The Effects of Cold Pressor-Induced Pain on PASAT Performance. Tapscott BE, Etherton J. Appl Neuropsychol Adult. 2015; 22(3):227-32. The Effects of Cold Pressor-Induced Pain on PASAT Performance. Tapscott BE1, Etherton J. Appl Neuropsychol Adult. 2015;22(3):227-32.