Introduction: Colitis falls under a group of disorders called inflammatory bowel disease (IBD) and marked with the presence of reactive oxygen species (ROS). The therapeutic target is to reduce inflammation and ROS. Aim: This study investigated the anti-inflammatory and anti-oxidative properties of Triticum aestivum on acetic acid-induced colitis in Wistar rats. Materials and Methods: Male Wistar rats (n = 12, 150-200g) were randomly divided into 4 groups as follow; group I(negative control) distilled water, group II- acetic-acid (6%) induced colitis (positive control), group III- acetic-acid induced colitis + mesalamine (400mg/kg) orally and group IV-aqueous extract of common wheat (200mg/kg) orally for three weeks. Ulcerative colitis was induced on day 1 of the study using acetic acid via anal injection, and colitis was confirmed on day 3 by the presence of bloodstains in the stool counts. The rats were euthanatized (ketamine) on days 7 and 21. The colon tissue was harvested and homogenized. Activities of superoxide dismutase (SOD), malondialdehyde(MDA), glutathione(GSH), and expression of interleukin-6 (IL-6) was evaluated. Results: Data were expressed as mean ± SEM, analyzed using one-way ANOVA, with P<0.05. Triticum aestivum significantly decreases the MDA level in group IV on days 3 (8.9180±0.574) and 7 (9.531±0.133) when compared with group III on days 3 (12.950±0.457) and 7 (12.360±1.414) and group II on days 3 (13.380±0.501) and 7 (9.876±0.633). It also reduces the SOD levels and significantly increases the GHS level. A Significant decrease in interleukin-6 expression was observed in group IV on days 3(7884±77.2) and 7(5425±384.3) when compared with group II on days 3(10600±240.3) and 7 (8584±495.4).  In conclusion, aqueous extract of Triticum aestivum possesses anti-inflammatory and anti-oxidative properties in Wistar rats induced with colitis. Keyword: Colitis, Anti-inflammatory markers, Triticum aestivum, anti-oxidative