Plasma membrane ion channels are important for cell homeostasis with their combined properties often defining the function of that cell; however, for white fat adipocytes (WFA) the expression of ion channels and their associated roles remain unclear. Given the importance of chloride channels in WFA membrane potential  (Pulbutr et al., 2007; Bentley et al., 2014), our aim was to identify these at the molecular level. We explored this issue in adipocytes isolated from different adipose depots of adult Wistar rats, CD-1 mice, as well as in a common adipocyte cell line model: 3T3-L1. Animal care and experimental procedures were carried out in accordance with the UK Home Office Animals (Scientific Procedures) Act (1986) and were locally approved. Deepseq revealed the expression of various chloride channels isoforms in the visceral WFA of adult male rat epididymis. Among these, putative plasma membrane chloride channels were the volume regulated chloride channels Lrrc8a/b/c/d and Ttyh2/3, and the calcium-activated chloride channel, Ano1.  Relative expression was confirmed by RT-qPCR in WFA from visceral (rVWFA), mesenteric (rMWFA), perirenal (rPWFA) and subcutaneous (rSWFA) fat depots of rats, mouse subcutaneous (mSWFA) as well as differentiated 3T3-L1 cells. Statistical significance as p<0.01, to account for repeated use of data , was determined by ANOVA with Dunnet’s multiple comparison test relative to rSWFA for between depots, and relative to Ano1 for within depots. Data are given as ratios (95% Confidence intervals, number of determinations) relative to rSWFA or Ano1. Comparison between depots showed no difference in expression for Lrrc8a, Lrrc8c, Lrrc8d, Ttyh3 and ANO1 relative to rSWFA , whereas for rMWFA and rPWFA Lrrc8b was expressed by 4.9 (2.5 to 13, n=5) and 3.3 (1.6 to 9, n=5) fold greater, respectively. For 3T3-l1 cells, Lrrc8c was expressed at 2% (1 to 57, n=4)  of that seen in rSWFA. Comparison within depots showed that for rVWFA and rMWFA, Lrrc8a was expressed greater than Ano1by 7.4 (3.3 to 43, n=5) and 9 (5.1 to 18, n=5) fold respectively. In pSWFA; Lrrc8a, Lrrc8c and Ttyh2 were all expressed greater than Ano1 by 11 (5.7 to 92 (90%) , n=5), 6.9 (3.2 to 56 (90%) , n=5) and 7.5 (3.9 to 61, n=5) fold, respectively. Whereas for  rSWFA and mSWFA only Lrrc8c was expressed greater by 9.6 (2 to 174 (90%) , n=5) and 73 (11 to 173, n=5) fold, respectively. For 3T3-l1 cells, Ttyh2 was expressed at ~2000% (CI interminable, n=4). Our results show that the expression levels of  chloride channel related genes differ both between adipose depots and within 3t3-L1 cells. The Lrrc8x chloride ioh channel family had the greatest expression. The consequences of these expression profiles for depot-dependent adipose function are being investigated.