Adopting a healthy lifestyle is paramount to the prevention of cardiovascular disease. In recent years there has been a steep increase in terms of available strategies for health promotion, ranging from technology-driven to more naturalistic and holistic approaches. Meditation is one of such strategies, with recent studies highlighting a wide range of benefits, including improvement of cardiovascular physiology. However, few studies have addressed the role of microcirculation on the cardiovascular response to meditation. Our aim was to explore the effect of a single meditation session on the cutaneous microcirculation in healthy subjects. A group of twelve subjects (38.9 ± 17.7 y.o.) of both sexes, participated in this study after giving informed written consent. None of the subjects had previous experience in meditation practices and were properly instructed prior to carrying out the experimental procedure, which was carried in a temperature-controlled room. Subjects performed the procedure while seated and following a 10 min stabilization period. The procedure consisted of three phases – 2 min baseline (phase I), 10 min of focused attention meditation (phase II) and 2 min recovery (phase III). Cutaneous microcirculation was assessed on a randomly chosen earlobe with a photoplethysmography sensor, which quantified local blood flow. The raw blood flow signal was decomposed into its spectral components with the wavelet transform – cardiac, respiratory, myogenic, sympathetic, endothelial dependent and independent of NO. Statistical comparisons between phases were carried out with the t-test for related samples (p<0.05). During phase II no significant change in blood flow was observed. Similarly, the cardiac, respiratory, myogenic, sympathetic and endothelial NO-dependent activities also remained stable during this phase. However, the endothelial NO-independent activity decreased significantly during phase II, and remained significantly lower than baseline during phase III. These preliminary results suggest that a single meditation session might influence the mechanisms regulating microcirculatory perfusion.