Recent studies have suggested high-intensity intermittent exercise reduces body weight and increases cardiovascular fitness and insulin sensitivity in untrained individuals [1-3]. Little is known whether intermittent compared to continuous exercise differentially affects subsequent gastrointestinal and appetite response as well as substrate utilisation. This study aimed to investigate the effect of high intensity and low intensity intermittent and continuous cycling on gastric emptying rate, appetite, blood glucose and substrate utilisation in the hours following exercise and the consumption of a standardised semi-solid meal. Twelve healthy males (Mean ± SD; age 30 ± 6 years; height 179 ± 8 cm; body mass 79 ± 9 kg; VO2peak 38 ± 6 ml/kg/min) completed four 60 min cycles. These were either at 60% VO2peak (HIGH) or 40% VO2peak (LOW) in a continuous (CON) or intermittent (INT) manner. INT consisted of 20 X 1 min exercise bouts followed by 2 min rest. INT and CON at each intensity were matched for total work output and were completed in a randomised order after an overnight fast. Gastric emptying rate was measured for 2 h post-meal using the 13C-breath method. Blood glucose was measured at baseline, mid-exercise, post-exercise, pre-meal and at 30 min intervals following ingestion. Appetite was assessed at regular intervals throughout the trials and substrate utilisation was measured at baseline and every 30 min after exercise. No differences in fat and carbohydrate oxidation (P>0.05) between trials were observed. Blood glucose was greater on LOW-CON compared to LOW-INT post-exercise (4.8 ± 0.5 v 4.4 ± 0.5 mmol/L: P=0.006) and 30min post-meal (7.6 ± 1.0 v 7.0 ± 1.0 mmol/L: P=0.013). Blood glucose was greater on HIGH-INT compared to HIGH-CON mid-exercise (4.9 ± 0.4 v 4.3 ± 0.5 mmol/L: P= 0.027), but lower 30 min (6.5 ± 0.9 v 7.5 ± 0.9 mmol/L) and 60 min post-meal (4.8 ± 0.4 v 5.4 ± 0.7 mmol/L: P=0.011). No differences for gastric emptying lag-phase and half-emptying time of the test meal were observed (P<0.05), however, Delta Over Baseline (DOB) values were lower (P < 0.05) on HIGH-INT compared to HIGH-CON from 30-120 min after ingestion (Figure 1). Hunger and Prospective Food Consumption were lower between 60-75 min during the HIGH-INT compared to HIGH-CON (P<0.05). The results of this study demonstrate that high intensity intermittent cycling may delay subsequent gastric emptying rate of a semi-solid meal and reduce blood glucose compared to high intensity continuous cycling without significantly increasing appetite or effecting substrate utilisation within a 2 h recovery period. The mode of exercise appears to have little impact on these markers when performed at a low intensity.