Objectives: Metabolomics is has become as an established strategy in the exploration of various stimuli effects. We have recently shown that intense physical exercise can extensively modify the urinary metabolome in human subjects (Ref 1). We have now investigated whether physical training could induce changes at the resting level and after a physical test. Methods: Fourteen men performed 2 sets of sprint tests separated by a long sprint training program (6 sessions performed in two weeks). Urine specimens were collected at rest and 30 minutes after both sprint tests. Samples were analysed by 1H NMR spectroscopy and multivariate statistical technique (i.e. Principal Component Analysis and Partial Least Square Discriminant Analysis). Results: Preliminary results show clear separation of the urine profiles obtained from specimens collected before vs. 30 min after the tests and from specimens collected at rest, before and after the training program. Discussion: Our results shown that metabolomics could provide information on the changes that physical training could induce and seems to be a promising tool to make a deep insight into physiologic states of athletes. We now need to further explore the spectrum profiles of the metabolome in order to indentify the molecules involved.