The development of neurons from induced pluripotent stem cells (iPSCs) provides research models which offer the potential of being more representative of human cells than existing animal and cell-line based models. The potential of iPSC-derived cells is currently undermined by two major drawbacks; the length of time required to differentiate and culture them and an often-immature phenotype. The ability to produce more phenotypically relevant cells in a shorter time-frame would be a major boon and would allow iPSC-derived cells to truly achieve their potential as a game-changing research platform. Here we present data obtained from Axol’s Human iPSCs-derived cortical and sensory neurons demonstrating a more rapid maturation and more physiological-relevant phenotype than produced via conventional cell-culturing protocols. The maturation and functionality of the neurones was assessed from their morphology, PCR, immunocytochemistry (ICC) and electrophysiology using calcium imaging and multielectrode (MEA) arrays. For the sensory neurons, Axol’s Human iPSC-derived sensory neuron progenitors (ax0055) were thawed and plated. After Mitomycin C, treatment the cells were placed in an Axol media (ax0058) to mimic the in vivo environment during development and were cultured up to Days In Vivo 32 (DIV32). Morphological changes were apparent in cells treated with a sensitisation cocktail by DIV8 ± 1.5 (SEM, n=4) and the improved maturity and functionality was confirmed by PCR, ICC, MEA recording and calcium imaging compared to control cells by DIV20 compared to 4-7 weeks using conventional tissue culture. For the cortical neurones, Axol’s Human iPSC-derived neuronal stem cells (ax0016) were thawed, plated and differentiated into cerebral cortical neurones. Maturation was accelerated through changing media composition, co-culture and the transfection of transcription factors. The maturation and functionality of the neurones was assessed from their morphology, ICC and electrophysiology using calcium imaging and MEA arrays. Morphological differences were apparent from DIV9.2 ± 1.1 (SEM, n=5). PCR confirmed an increase in expression levels of several key cortical markers by DIV3 (1.74 – 2.69-fold increase in TUJ1, MAP2, TBR1 & PAX6, n=3). Accelerated functional maturity was confirmed by the earlier appearance of spontaneous spike firing (DIV16 vs DIV34; 178.3 ± 6.3 vs 57.6 ± 7.5 spikes/min), burst firing (DIV16 vs DIV34; 8.1 ± 0.9 vs 0.90 ± 0.48 bursts/min) and synchronised burst firing (DIV35 vs DIV64+), compared to control conditions. With the methods described here Axol Bioscience demonstrate the ability to produce more functionally relevant cortical and sensory neurones in a shorter time-frame than currently typical. These findings are should be translatable to other cell-types and this will greatly improve the utility of iPSC-derived cells to research and industry.