Function of Ca2+-transporting systems could depend on animals’ age. The age related features of interaction between Ca2+-transporting systems (IP3Rs, RyRs and mitochondrial Ca2+-uniporter) were investigated in rat hepatocytes. Experiments were performed using isolated hepatocytes by lidase digestion. Isolated hepatocytes were incubated with saponin for permeabilisation. Concentrations of membrane-bound Ca2+ were measured using chlortetracycline. All rats were divided on 3 different groups: young (age < 6 months, weight 0.05-0.06 kg), adult (6 months, weight 0.18-0.2 kg) and old rats (18-24 months, weight 0.45-0.50 kg). It was shown that IP3 (10 μM) decreased the membrane-bound Ca2+ content in permeabilized hepatocytes of both young (by 27.72±9.20%; Ρ<0.05, n=9) and old rats (by 20.47±8.23%, Ρ<0.05, n=13), and increased the membrane-bound Ca2+ content in permeabilized hepatocytes in groups of adult rats by 15.99±5.29% (Ρ=0.05, n=10). Ruthenium red (1μM) did not elicit significant changes in the membrane-bound Ca2+ content in hepatocytes of young and old rats, but decreased it by 22.09±7.58% (Ρ=0.05, n=7) in groups of adult rats. Simultaneous application of IP3 and ruthenium red didn’t cause statistically authentic change of membrane-bound Ca2+ content in all groups. We also showed that ryanodine (5 nM) decreased concentration of the membrane-bound Ca2+ in permeabilized hepatocytes of young rats by 28.16±5.54% (Ρ=0.05, n=10), old rats – by 17.95±8.66% (Ρ<0,05, n=13), but increased the membrane-bound Ca2+ by 29.94±7.49% (Ρ<0.05, n=8) in groups of adult rats. In the presence of both ruthenium red and ryanodine content of membrane-bound Ca2+ in permeabilized hepatocytes was decreased by 25.52±11.03% (Ρ<0.05, n=10) in groups of young rats. Simultaneous presence of ryanodine and ruthenium red at incubation medium caused increase of membrane-bound Ca2+ by 17.41±7.38% (Ρ<0.05, n=13) in groups of adult rats. There was no statistically authentic change of membrane-bound Ca2+ in permeabilized hepatocytes of old rats in the presence of both ruthenium red and ryanodine. Therefore, we observed that (1) both IP3 and ryanodine evoke age-dependent changes of membrane-bound Ca2+ content, which have similar character; (2) ruthenium red alone affected membrane-bound Ca2+ content in groups of adult rats only. The age-dependent changes of membrane-bound Ca2+ content in hepatocytes were also evoked by simultaneous application of ryanodine and ruthenium red. Thus, we conclude that there is an age-dependent interaction between Ca2+ uniporter and RyRs of hepatocytes, but the interaction between Ca2+ uniporter and IP3Rs is not age-dependent.