Extracellular Ca2+ influx is critical to many functions of white fat adipocytes (WFA), such as growth, lipolysis and adipokine secretion (1). Pharmacological evidence suggests that this Ca2+ influx is mediated in part by L-type voltage-gated calcium channels, VGCCs (2). However, due to technical constraints, electrophysiological evidence for the presence and identification of these VGCCs in WFA is still lacking. To address this problem we have utilised RT-qPCR and Western blotting to determine the effect of adipose-depot, species, age, and gender on VGCCs expression in WFA. WFA were isolated from Sprague-Dawley rats and CD-1 mice. For subcutaneous adipose depots, we used inguinal fat and for visceral, epigonadal: epididymal for male and periovarian for female. In addition, 3T3-L1 cells, an established model of mouse subcutaneous WFA were used for comparison (1). Data, mean±S.E.M., are shown as rank order of relative expression or as ratios, their statistical significance as p<0.05 was determined by ANOVA with Holm-Sidak’s multiple comparison test or One sample-t-test respectively. All cells tested expressed CaV1.1, CaV1.2, CaV1.3 and CaV3.1, but not CaV1.4. The amount for each individual isoform in epididymal WFA was similar to that for inguinal fat. The expression llevel for each VGCC was also similar between epididymal and periovarian WFA. However, expression significantly varied between isoform with a rank order of CaV1.2 > CaV1.3 >= CaV3.1 >=CaV1.1, relative expression levels to CaV1.2 were 0.3±0.04, 0.16±0.04 and 0.02±0.002 respectively (n=16). We saw no difference in the expression profiles between rat and mice. The expression of Cav1.2 was 5 times larger in juvenile (p16-p21) than adult rats, however they showed similar levels of expression for the other VGCCs studied (n=6-16). CaV1.2 and CaV3.1 expression levels significantly decreased upon 3T3 differentiation: 1.2±0.09 to. 0.26±0.05 and 1.2±0.14 to 0.22±0.05 respectively, much lower values were seen in mouse subcutaneous WFA: 0.015±0.003 for CaV1.2 and 0.002±0.0005 for CaV3.1 (n=4-5). Conversely, subcutaneous fat had the highest expression of CaV1.3; 23±6.8 vs. 1.2±0.25 for 3T3s. CaV1.1 had similar expression levels. Western blots revealed that in WFA CaV1.2 has a mw >290 kD, larger than that expected, 240-250 kD(3). A fact possibly explained by extra exons at the N and C-termini as revealed by 2nd generation Deep Sequencing. Our data shows that Cav1.2 and Cav1.3 are the dominant isoforms of VGCCs expressed in adult murine WFA, with similarity in expression levels between adipose depots, gender and species. CaV1.2 and CaV3.1 have a greater expression in juveniles or undifferentiated cells, which suggests a role for these VGCC isoforms in multipotency. Cav1.2 appears to have an isoform unique to WFA.