High blood pressure (hypertension) indicates the deterioration of the set points of metabolic fitness and is one of the most frequent metabolic risk factors in Western countries. Pharmacological inhibition of angiotensin 2 (ANGII) by ACE inhibition action may have undesirable side effects, particularly, in the situation when one wants to exploit the sympatholytic and angiogenic benefits of physical therapy. Limited studies investigated ANGII levels related to exercise intensity and duration, but it seems to be that there is only an increase above with exercise intensity above the anaerobic threshold. The aim of this research is to detect changes in human ANGII levels after exercises of different intensities at or above the anaerobic threshold. On three separate occasions fit healthy male subjects (n=10, age: 29.6±2.1 year, length: 180.9±7.6 cm, mass: 76.1±5.6 kg) performed a maximal incremental exercise tests, a 12-minutes test at an intensity were they reached their aerobic threshold intensity and a 3-minutes test their ventilator compensation point intensity. Expired air was measured breath-by-breath, blood samples were taken before and 0, 3, 6 and 12 minutes after exercise and subject were screened for the ACE I-allele polymorphism. Blood was measured on lactate concentration and serum on ANGII levels. Values are means ± S.E.M. Direct after the 3-minutes cycling test there was a 1.8-fold increase in ANGII serum levels compared to resting levels (paired T-Test, 3.20±0.83 vs. 5.75±0.81 pg/mL, p=0.001) and after the 12-minutes test there was a 1.2-fold increase (paired T-Test, 4.4±1.47 vs. 5.5±1.30 pg/mL, p=0.24). ANGII levels were not affected after the maximal exercise test. 12 minutes after maximal exercise subjects with the ID/DD genotype had a significant lower ANGII serum level compared to subjects with the II genotype (ANOVA, 1.7±1.0, 9.6±5.5, p<0.05). ANGII levels were not correlated with blood lactate levels. ANGII could be detected after a non-maximal high intensity exercise. The data indicate that variability in the response of the major vasoconstrictor, ANGII, in serum after exercise is related to the ACE genotype dependent but not blood lactate. This research was funded by the European Commission through MOVE-AGE, an Erasmus Mundus Joint Doctorate programme (2011-0015).