Concerning the variability of effectiveness of essential oils from different species of Nepeta genus on CNS including analgesia, this study was designed to evaluate antinociceptive and anti-inflammatory effects of essential oil of Nepeta pogonosperma Jamzad et Assadi (NP). Air-dried aerial parts of NP were hydrodistillated and GC-MS analysis of obtained essential oil was conducted. Total 24 male Wister rats (225±25gm) were taken care according to international guidelines on use of laboratory animals and studied. Experimental rats received 50, 100 and 200mg/kg of NP essential oil and control rats received equal volume (2ml/kg) of normal saline intraperitoneally (n=6/group). Antinociception was assessed by tail flick test (D’Amour and Smith, 1941) followed by formalin test (50 μl of 2% formalin, subcutaneously) using a modification of original formalin test protocol (Dubisson and Dennis, 1997). Euthanasia was done by di-ethyl ether (99%, 10-12ml) followed by decapitation (Zschenderlein et al. 2011) and anti-inflammation was measured by water plethysmometry (Fereidoni et al. 2000). Data were mean±SEM and analyzed by ANOVA with Tukey’s post-hoc test. NP essential oil consisted of 4aα,7α,7aα nepetalactone (14.5%) and 1,8 cineole (31.2%) mainly. In tail flick test, 50mg/kg (10.25±8.06 vs. 11.04±5.9%) did not produce any analgesia, but 100 (26.99±2.79%; p<0.01) and 200 (43.98±7.73%; p<0.001) mg/kg reduced pain significantly. In early phase of formalin test, all doses reduced jerking frequency/5min (50mg/kg=78.66±20.67; 100mg/kg=64.5±15.47; 200mg/kg=30.83±7.16; control=201.33±14.01; p<0.001, in all doses), flexing seconds/5min (50mg/kg=62.5±20.24; 100mg/kg=65.33±18.31; 200mg/kg=50.5±21.14; control=148±21.57; p<0.05, p<0.05, p<0.01, respectively) and licking seconds/5min (50mg/kg=28.83±17.97; 100mg/kg=23.66±7.56; 200mg/kg=4.33±3.94; control=82±8.95; p<0.05, p<0.001, p<0.001, respectively). Again, in late phase of formalin test, all doses reduced jerking frequency/5min (50mg/kg=45.79±7.54; 100mg/kg=34.42±7.75; 200mg/kg=38.48±7.08; control=112.03±10.53; p<0.001, in all doses), flexing seconds/5min (50mg/kg=60.59±21.53; 100mg/kg=76.07±27.69; 200mg/kg=40.94±26.19; control=224.06±20.43; p<0.001, p<0.01, p<0.001, respectively) and licking seconds/5min (50mg/kg=12.83±3.22; 100mg/kg=18.46±4.78; 200mg/kg=5.66±1.66; control=33.03±3.83; p<0.01, p<0.05, p<0.001, respectively). Moreover, all doses reduced paw edema in % of control (50mg/kg=0.21±0.04, nonsignificant; 100mg/kg=0.13±0.02, p<0.01; 200mg/kg=0.2±0.03, p<0.05). This study reveals that NP essential oil may minimize both acute and chronic forms of nociception and may have potent role against inflammation.