Arginine vasopressin (AVP) release is known to be stimulated by hypovolemia as well as osmotic challenge such as dehydration and chronic salt loading. We have generated a transgenic rat that expresses the AVP-eGFP fusion gene in the paraventricular (PVN) and the supraoptic nuclei (SON) of the hypothalamus. Our previous studies revealed that acute and chronic osmotic stimuli up-regulated the expression of the AVP-eGFP fusion gene in the magnocellular neurons of the PVN and the SON. Here, we demonstrated that polyethylene glycol (PEG)-induced hypovolemia was able to cause the up-regulation of the AVP-eGFP gene expression in the PVN and the SON in the transgenic rats. Intraperitoneal (ip) administration of PEG induced isotonic hypovolemia. The intensity of eGFP fluorescence in the PVN and the SON were significantly increased 6 and 24 hours after ip administration of PEG in comparison with controls. Interestingly, the intensity of eGFP fluorescence in the parvocellular division of the PVN was also increased significantly. The present study revealed that PEG-induced hypovolemia caused a significant up-regulation of the AVP-eGFP fusion gene expression in the magnocellular and parvocellular neurons of the PVN and the SON in AVP-eGFP transgenic rats.
37th Congress of IUPS (Birmingham, UK) (2013) Proc 37th IUPS, PCC126
Poster Communications: Arginine vasopressin (AVP)-eGFP expression in the hypothalamus after hypovolemia in AVP-eGFP transgenic rats
Y. Ueta1, M. Ohno1, T. Ishikura1, T. Maruyama1, H. Hashimoto1, T. Matsuura1, J. Ohkubo1, M. Yoshimura1, H. Yamashita1
1. Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
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