Reactive oxygen species are known to play an important role in the inflammatory response. The cutaneous inflammatory response produces a visible erythema that can be quantified with Laser Doppler Flowmetry (LDF). Our aim was to test the efficacy of our antioxidant-containing formulations to reduce the erythema response to a topically applied vasodilator. 12 subjects (26.6±6.4 years old) participated in this study after informed consent. Four 9 cm2 areas were marked on the volar surface of both forearms. Three of these areas were topically treated with sunscreen formulations containing or not antioxidant nanoparticles (F1, F2, F3) twice-daily, for 7 days, with the fourth remaining non treated. The formulations’ composition is shown in Table 1. After this period, ethyl nicotinate (EN) was applied on each site to induce an inflammatory response and skin perfusion was measured for 20 minutes with LDF (PF 5010 system, Perimed, Sweden). Area under the curve (AUC) and the slope of the curve on the hyperemic phase were chosen as comparison parameters between sites’ responses. The LDF signal was decomposed with the wavelet transform into its main components (cardiac, respiratory, myogenic, sympathetic, endothelial). Wilcoxon signed-rank test was used as comparative statistics (p<0.05). Significant differences were found for the curve slope between the three formulations. Our results indicated that the presence of gelatin nanoparticles decreased the intensity of the erythema caused by EN when compared to F1. No significant differences were found for the components’ activities between control and treated sites or between the sites themselves. Our results suggest that topical formulations containing antioxidants show potential to inhibit the cutaneous erythema response.