Cadmium is an extremely toxic metal commonly found in industrial work places. It acts as a catalyst in forming reactive oxygen species1. It increases lipid peroxidation, in addition it depletes antioxidants, glutathione and protein-bound sulfhydryl groups2.Cadmium is known to affect various organs like kidney, liver, bone and testis in human beings and experimental animals3. The present study was aimed to study the anti-oxidative role of beta carotene against cadmium chloride induced oxidative stress on rat testis. The present study was conducted following approval from Institutional Bioethical Committee and strict internationally accepted guidelines, for the usage of animals in experimental study were followed. Inbred adult male albino rats of wistar strain weighing 200-250g were used in the present study. Rats were divided into following groups ,Control group(Gr.I) rats were treated with 0.9% Saline intraperitonally, Beta carotene(10mg/kg bw) treated(Gr.II) for 30 days orally .The untreated group(Gr.III) were injected with single dose of 1 mg /kg body weight cadmium chloride intraperitonally. Pretreated group (Gr.IIIA)-Beta carotene (10mg/kg body weight) was orally administered for 30 days prior to the exposure to 1mg/kg body weight of cadmium chloride. In all the groups, rats were sacrificed15 days after the final cadmium chloride or saline administration. The data was expressed as Mean + SD. The difference between groups was compared for statistical significance by student t test with the level of significance set at P<0.05. Exposure to cadmium chloride(Gr.III) lead to significant(P<0.001) decrease in the epididymal weight (0.312±0.06)& sperm count(242.83±8.71) compared to Gr.I(0.502±0.06, 892.83±8.90) & Gr.II(0.498±0.02, 887.36± 8.14)and Standard Tubular Diameter(STD)(414.26±23.4)and Standard Epithelial Height (SEH)4( 31.56±8.13) was also low in Cadmium treated group(Gr.III) compared toGr.I ( 517.42±22.6, 68.24±4.36)& Gr.II(511.68±31.6,63.81±3.87). Photomicrograph of rats in Gr.I & Gr.II showed normal architecture of testis. Photomicrograph of rats treated with cadmium chloride showed the destruction of the seminiferous tubules. But, rats pretreated with Beta carotene prior to cadmium administration showed an significant (P<0.001) increase in epididymal weight(0.492±0.09) & sperm count(801±5.12)compared to cadmium treated group. Standard Tubular Diameter (508.36± 23.70) and Standard Epithelial Height (63.84±7.84) was also significantly (P<0.001) high in beta carotene pretreated group. Photomicrograph of rats testis pretreated with beta carotene showed the almost normal testicular architecture. Thus the results of the present study showed the antioxidative role of beta carotene in ameliorating cadmium induced testicular damageKey Words: Cadmium, beta Carotene, sperm count, standard tubular diameter (STD)