KATP channels were originally discovered in cardiac myocytes. Since then they have been molecularly defined, e.g. it has been shown that they are comprised of four Kir6.1 or Kir6.2 and four SUR1 or SUR2A/B subunits. Substantial progress has elucidated their role in the physiology and pathophysiology of pancreatic beta cells and cardiac myocytes. However, the biochemical description of KATP channel complexes from native cells lags behind the many functional and physiological insights that the field has generated. Here we provide a detailed characterization of Kir6.2 and SUR1 proteins in different cell types of the heart. We employ blue native gel electrophoresis, glycan analysis, affinity purification and indirect immunofluorescence to demonstrate different species of KATP channels. Our results will be integrated with current knowledge on the trafficking and cell surface expression of heterologously expressed KATP channels.
University College London (2011) Proc Physiol Soc 24, SA17
Research Symposium: Biochemical Analysis of cardiac ATP-Sensitive potassium (KATP) channels
E. Arakel1, S. Brandenburg1,2, H. Zhang3, S. Lehnart2, C. Nichols3, B. Schwappach1
1. Department of Biochemistry I, Universitõtsmedizin G÷ttingen, G÷ttingen, Germany. 2. Heart Research Centre, Universitõtsmedizin G÷ttingen, G÷ttingen, Germany. 3. Department of Cell Biology and Physiology, Washington University, St Louis, Washington, United States.
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Where applicable, experiments conform with Society ethical requirements.