Myoelectric activity has been shown to increase during whole-body vibration (WBV) in a frequency (1,2) and amplitude (2) dependent fashion, which may be related to the propagation of vibration through the body. We have therefore explored the relationship between vibration transmission and muscle activity during WBV of different frequencies and amplitudes. Surface electromyography (EMG) was recorded from the Soleus (SOL) and Vastus Lateralis (VL) of 8 healthy men during isometric squat exercise (150° knee joint angle). 30s squats were performed on a WBV platform during 1.5mm and 3mm p-p amplitude vertical WBV of 20, 25, 30 and 35Hz (8 squats) in pseudorandom order interspersed with 60s rest. To quantify WBV transmission, vertical acceleration was measured via skin-mounted tri-axial ±10G accelerometers placed at the segmental centre of mass of the shank and thigh, and on the platform. Data were analysed by 2 way repeated measures ANOVA (amplitude vs frequency), and regression analysis to determine any relationship between segmental accelerations and EMG activity. Shank (p=0.003) and thigh (p=0.007) vertical acceleration were significantly greater at 3 than 1.5mm. There was a main effect of frequency on shank acceleration (p=0.001) with the highest at 25Hz (1.5mm amp: 1.41± 0.68; 3mm amp: 2.07±1.11; root mean square, RMS G; mean ±SD) and the lowest at 35Hz (1.5mm: 0.78 ± 0.49; 3mm: 1.08±0.84; RMS G). Shank was greater than thigh vertical acceleration (p<0.001), however only a weak relationship existed between these segment accelerations (R²=0.28, p<0.001). There was no significant effect of WBV frequency on SOL or VL RMS EMG amplitude, although SOL RMS EMG amplitude was greatest at 25Hz (1.5mm: 0.012±0.005; 3 mm: 0.015±0.007 V) and lowest at 35 or 30 Hz for 1.5 and 3 mm amplitude WBV respectively (0.008±0.004, 0.011±0.007 V). There was no relationship between shank acceleration and SOL RMS EMG amplitude (R²= 0.02,p=0.215), and a positive relationship (R²=0.36,p<0.001) between thigh acceleration and VL RMS EMG amplitude. Both shank and thigh acceleration were greater at the higher WBV amplitude but only shank acceleration exhibited WBV frequency dependency. There was significant attenuation of vibration between shank and thigh, presumably due to passive and active damping via joint compliance and muscle activation, respectively. There was significant inter-individual variation in segmental acceleration and muscle activity, which may be indicative of individualised strategies for vibration damping.