Introduction: The human body has evolved in Earth’s gravity (1Gz), effecting physiological development and daily locomotion. When exposed to extended periods of microgravity, physiological de-conditioning ensues, despite exercise being implemented to counter deconditioning. With further exploratory missions planned away from Earth’s 1Gz environment, more comprehensive countermeasures are required to protect the human body. The SkinSuit provides a potential integrative countermeasure against microgravity deconditioning by imparting a cumulative axial loading regime, however little research exists on the effects of reloading subjects in altered gravities. A range of ‘gravity loading’ was created to study the response to running, at self-selected speed. Methods: A healthy male (72kg; 1.70m; 26yr) volunteered for the study which received local ethical approval by PUCRS Research Ethics Committee. After familiarisation and a five minute resting state, the subject performed five minutes of running at a self-selected running pace on a treadmill at three randomised gravities, once with and once without the SkinSuit. In addition to normal 1Gz running, bodyweight suspension was used to unload the subject to simulate Martian (0.38Gz) and Lunar (0.16Gz) gravities; when worn the SkinSuit provided approximately an additional 0.8Gz of axial loading, thus creating a range of ‘gravity loading’ (0.16-1.8Gz). Respiratory responses, heart rate (HR), gait kinematics and ratings of subjective comfort (1-10), were reported for rest and the final minute of each run. Results: Respiratory responses were positively, linearly associated with gravity loading (r=0.94), with minute ventilation increasing from rest by 10.9 l.min-1 when running at normal 1Gz without the SkinSuit, to 31.3 l.min-1 when running at 1Gz with the addition of the Skinsuit (+0.8Gz). HR was not associated with loading, but was associated with self-selected running speed. In reduced simulated gravities i.e. Lunar, a change of gait was observed with a shift to ‘progressive jumping’. Greater need for movement control (4/10 vs.1/10) was reported when wearing the SkinSuit. Conclusions: In this case study a linear, positive, trend between ‘gravity loading’ and respiratory response was observed. When the SkinSuit (0.8Gz) was combined with the Lunar simulation (0.16Gz), approximating Earth’s 1Gz, the respiratory responses were nearly identical, indicating the potential role of gravity loading on metabolic cost.