The use of synthetic materials and biomaterials as part of tissue-engineered vascular devices, such as heart valves and vascular grafts, has long been the focus of research. However, despite the large availability one of the limits of these materials is the low patency and elasticity associated with the risk of trombogenicity. Tissue engineered biological grafts have been shown to have a more favourable outcome and better longterm patency than currently available prostetic grafts. A decellularization process that efficiently removes all cellular and nuclear material, while preserving the extracellular matrix (ECM) components, is required in order to prevent any immuno-mediated rejection that might arise from xenogenic or allogenic tissue implantation. The aim of this study is to optimize a decellularization protocol specific for different porcine tissues, with a view on recellularization with autologous thymus-derived Mesenchymal Stem Cells (MSC) to produce tissue-engineered scaffolds that can be used as a patch for cardiovascular repair. Porcine pericardium and pulmonary artery collected from piglet were treated with ionic detergent (SDS), non-ionic detergent (Triton) or enzymatic biologic agent (Trypsin), followed by incubation with nuclease solution.H&E histological analysis of decellularized matrices showed that, while either the Triton and the Trypsin treatment effectively removes the nuclear content from the pericardium specimens, the Trypsin is more efficient in preserving the extracellular tissue content. However, despite the slight tissue break caused by the Triton treatment, the MSCs growth after reseeding is not affected, as shown by the cell viability/cytotoxicity assay. Differently, the detergent treatment has proven to be more effective in removing the nuclear material on pulmonary artery. In particular, the nuclei content after non-ionic detergent treatment was almost absent compared to the one observed by treating the tissue with the ionic detergent SDS. Negligible effects were observed on the extracellular matrix components using either detergents. However, the Triton was more compatible with the cells growth, as shown by viability assay and H&E staining.This study identifies a tissue-specific decellularization method for native porcine pericardium and artery that maximized cellular elimination without compromising the integrity of the tissue, and the feasibility of MSCs growth on decellularized scaffolds either in a static and a dynamic model of cell culture, with this latter mimicking an in vivo scenario. Future studies involve the evaluation of the mechanical integrity and elasticity of the decellularized and recellularized tissues.