Purpose of the thesis is to determine and compare the level of motor skills using a standardized test of five youth football teams U11. A series of exercises using Unifittest 6-60, tests the player in four different exercises focused on the individual physical abilities. All teams will complete tests by the end of training, at which time they should exhibit the highest degree of fitness. We personally perfom all testing. The aim of our work was to measure and compare four league football teams and one district team using a test battery Unifittest 6-60 and to find out the level of motor abilities and skills. For this testing we used certified Unifittest 6-60, which we have slightly rearranged. The test included four disciplines. Players underwent scaphoid run of 4 x 10 m, then long jump from the place, sit-ups for 1 minute and finally we tested their endurance in pull-up.We got some somatic data from the players – this data included their height, their weight, the leg they naturally use for playing and the hand they use for writing. All measured data was processed and evaluated using both total and partial arithmetic mean. Standard divergence was also used. When creating graphs and boxplots we used Microsoft Office Excel and statistical program called R. For collecting data we used standardized methodology which provides high precision measurements and the possibility to compare the results with norms for that age group. For processing and interpreting data commonly available statistical methods were used. In mutual comparison the winner was AC Sparta Praha. The players showed on average the best results and with the exception of the discipline sit-up for 1 minute, they controlled the rest of remaining three disciplines and they deserve appreciation for the winners of our testing. As the weakest was for obvious reasons team SK Tochovice. In comparison of older and younger players in all four exercises, older players won but not always with a great distance. In two disciplines the results were very close, but for players born in the first half of the year were a bit better. This thesis has accomplished our aims. We enjoyed testing and work with promising footballers. We found out the difference between the league and the district team, which of the four major league clubs have the best conditions for training, base and how they hold up in our tests. We have to say that physical fitness and preparedness is very good. For evaluation we used the statistical program R and Microsoft Excel. We used the program R for Box plots illustrating the range of outcomes, then 25% and 75% quartiles, which enclose 50% of the results and median, which is depicted by a thick line. Microsoft Office Excel helped us to create tables and graphs.