Textural analysis performed using Gray Level Co-occurrence Matrix (GLCM) analysis is a contemporary mathematical algorithm applicable in quantification of cell and tissue structure. So far, it has been successfully applied in various biological and medical fields, including neurosciences. Some of the chromatin textural features may change as the result of various physiological and pathological processes, such as ageing, apoptosis and immune responses. On the other hand, the relationship between GLCM parameters and nuclear shape remains unknown. The aim of our research was to test the existence and strength of correlation between chromatin structural characteristics and nuclear envelope shape in a sample of hippocampal dentate granule cells. Brain tissue was obtained from 10 healthy male Swiss albino mice, and stained using Feulgen method for DNA visualization (figure 1). Digital micrographs of dentate gyrus structures were made in 8-bit gray scale format. Chromatin analysis on a sample of 100 dentate granule cells (10 per animal) was performed in ImageJ software and its plugins (National Institutes of Health, Bethesda, MD) directly from the micrographs. For each chromatin structure GLCM entropy (chromatin disorder), angular second moment (indicator of uniformity) and inverse difference moment (homogeneity) were determined. Nuclear envelope circularity as an indicator of nuclear envelope shape was calculated based on the nuclear area and perimeter. There was a statistically highly significant (p<0.01) negative correlation between circularity and angular second moment. Angular second moment increased as the circularity decreased and vice versa. No such relationship (p>0.05) was detected between circularity and other GLCM parameters. These results indicate that the two-dimensional roundness of nuclear envelope is strongly related to the uniformity of chromatin structure. This is one of the first studies to test the connection between nuclear shape and chromatin structure in this manner. The detected correlation may be explained by the specific events occurring in nuclear lamina in physiological conditions.