Background: Plant cyclotides are chemically stable and could be beneficial for medicinal application if they possess such properties. Cajanus Cajan exhibits anti-diabetic activity; however whether its cyclotide fraction also exhibits anti-diabetic effects is unknown. The present study was thus designed to determine the anti-diabetic effect of Cajanus Cajan Cyclotides (CCC) in type 2 diabetic rats. Method: CCC fraction was obtained by solvent extraction and peptide purification. Adult male wistar rats weighing 180-200 g were divided into six groups (n=7). Group 1 animals served as control and were untreated. Group 2-6 were induced with diabetes. Group 3 was administered with metformin for 14 days after induction of diabetes, Groups 4-6 were administered with CCC 10, 20 and 40 mg/kg respectively for 14 days after induction of diabetes. Diabetes was induced in rats by 3 weeks high sucrose diet followed by a single i.v low dose of streptozotocin (50mg/kg). Markers of glycaemic control, liver function test, liver histology, immunohistochemistry and biochemical markers were determined. Result: Diabetic presentation in rats included a significant increase in fasting blood glucose from 87.5±4.95 to 106.5±9.2 mg/dl, increased area under the cover for oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) from 9488±668 to 11693±301.8 and 4020±520 to 5940±485 mg/dl min. respectively; significant increases in plasma triglyceride from 61.64±4.79 to 146.6±15.22 mg/dl when compared with control. Treatment of diabetic animals with CCC at various doses mitigated diabetic presentations which were associated with up regulation of hepatic IRS-1 and skeletal muscle GLUT 4 expressions.  Conclusion: CCC possesses remarkable anti-diabetic activity which was associated with improved insulin signalling.