Macrophages of the innate immune system are increasingly recognised as highly plastic, multifunctional cells that can influence multiple physiological and pathophysiological states. In the heart they support homeostatic functions, contribute to disease progression and play multiple roles in reparative and regenerative processes following tissue damage. Understanding the heterogeneous populations of cells that contribute to these diverse functions is crucial to facilitating beneficial, and limiting adverse, cardiac outcomes. Characterisation of precise populations of cardiac macrophages and related cells remains incomplete in vertebrate models capable of endogenous regeneration, such as adult zebrafish, and yet the ability to identify sub-populations of cells and understand their localisation in the heart would greatly facilitate the study of their precise functions. We have used a combination of transgenic lines to identify four distinct immune cell populations in the zebrafish heart. Macrophages are present in developing cardiac tissue from 2 days post fertilisation and are likely seeded from both primitive and definitive derived cells and correct seeding requires csf1ra. In adult hearts, macrophages and dendritic cells are differently distributed and can be distinguished via expression level of the perforin mpeg1.1. Following injury, tissue resident macrophages rapidly proliferate and exhibit limited pro-inflammatory activation, similar to what has been observed in neonatal mice. This new understanding of innate immune cell populations in the heart of regenerative adult zebrafish sheds light on the composition of a pro-regenerative cardiac microenvironment.
Regenerating the Cardiovascular System (University of Oxford, UK) (2023) Proc Physiol Soc 52, SA02
Research Symposium: Defining immune cell populations in the zebrafish heart
Rebecca Richardson1,
1University of Bristol Bristol United Kingdom,
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Where applicable, experiments conform with Society ethical requirements.