Adrenomedullin (AM) is a 52-amino acid peptide with important functions in cardiovascular regulation. AM has two specific receptors formed by the calcitonin-receptor-like receptor (CRLR) and receptor activity-modifying proteins (RAMP2, RAMP3), known as AM1 and AM2 receptors, respectively. In addition, AM has affinity for the calcitonin-gene-1 related peptide receptor (CGRP1), composed of CRLR+RAMP1. In brain, AM and their receptors are found in localized areas, including cerebellum. We found by quantitative autoradiography an increase in cerebellar AM density binding sites during hypertension, suggesting a role for cerebellar AM-system in blood pressure regulation. Thus, we assessed whether there is an altered expression of AM and its receptor components in the cerebellar vermis of male adult spontaneously hypertensive rats (SHR) and normotensive control Wistar Kyoto rats (WKY), of 8 and 16 weeks old. Animals were euthanized by decapitation; the cerebellar vermis was dissected, placed in lysis buffer, homogenized and centrifuged. The expression of AM, RAMP1, RAMP2, RAMP3 and CRLR was performed by Western blotting. The experiments were conducted according to good practice for the management of laboratory animals of the Bolivarian Republic of Venezuela and the approval of Animal Ethical Committee of the School of Pharmacy. Values are expressed normalized to β-actin expression as means ± S.E.M., compared by ANOVA. Our findings demonstrate a higher expression of CRLR (8 weeks vs. 16 weeks: WKY= 1.0±0.09 vs. 4.1±0.2 and SHR= 1.0±0.08 vs. 5.1±0.2), RAMP1 (8 weeks vs. 16 weeks: WKY= 1.0±0.03 vs. 4.1±0.1 and SHR= 1.0±0.09 vs. 5.1±0.2) and RAMP3 (8 weeks vs. 16 weeks: WKY= 1.0±0.03 vs. 4.1±0.2 and SHR= 1.0±0.09 vs. 5.1±0.1) in cerebellum of 16 weeks old compared to 8 weeks old rats, both in WKY and SHR rats. While, AM and RAMP2 expression was not altered with rat age. In addition, we found a higher CRLR, RAMP1 and RAMP3 expression and a reduced expression of AM and RAMP2 in the cerebellar vermis of SHR rats when compared with WKY rats, both in 8 and 16 week old rats (AM, 8 weeks old= WKY vs. SHR: 1.0±0.08 vs. 0.6±0.02; 16 weeks old: 1.0±0.09 vs. 0.55±0.03) (RAMP2, 8 weeks old= WKY vs. SHR: 1.0±0.09 vs. 0.55±0.02; 16 weeks old: 1.0±0.09 vs. 0.7± 0.02) (RAMP1, 8 weeks old= WKY vs. SHR: 1.0±0.07 vs. 1.45±0.08; 16 weeks old: 1.0±0.08 vs. 1.55±0.07) (RAMP3, 8 weeks old= WKY vs. SHR: 1.0±0.01 vs. 2.45±0.12; 16 weeks old: 1.0±0.08 vs. 1.55±0.05) (CRLR, 8 weeks old= WKY vs. SHR: 1.0±0.07 vs. 1.53±0.09; 16 weeks old: 1.0±0.07 vs. 1.45±0.08). Our findings demonstrate an age dependent expression of cerebellar AM system which is deregulated during hypertension, suggesting a novel functional role of cerebellar AM in the regulation of blood pressure.