Mechanisms of coupling between Ca2+-released and mitochondria Ca2+ uptake in secretory cells is interesting problems of modern cell physiology. The aim of this study was examine correlation between mitochondria and Ca2+-released channels in secretory cells of Chironomus plumosus L. larvae salivary glands. Experiments were done on isolated salivary glands that were incubated with saponin (0.1 mg ml-1, 10 min) for cell permeabilisation. After that, saponin-treated glands were incubated during 30 min at 25° C in control solution that contained (mM): 15.3 NaCl, 129.94 KCl, 0.35 Na2HPO4, 0.44 KH2PO4 and 5.55 glucose; pH 7.0. Other components (Ins(1,4,5)P3, ryanodine, ruthenium red) were added to this control solution. Changes in common calcium content in gland tissue were measured using the metallochromic dye arsenazo III. Changes of membrane-bound Ca2+ were measured using chlortetracycline. This method is based on property of chlortetracycline to fluorescence in hydrophobic environment after binding Ca2+. So, fluorescence changes of complex chlortetracycline-Ca2+ inside the membranes reflect shifts of ionized Ca2+ concentration close to membrane. The simultaneous presence of ruthenium red (10-5M) and Ins(1,4,5)P3 (10µM) in incubatium medium did not cause any statistically significant changes in common calcium content in comparison with control medium. In contrast to simultaneous presence of ruthenium red (10-5M) and ryanodine (5nM) evoked statistically significant (p=0,023, n=6) decreased of calcium content in comparison with control medium by 37.65±9.65 %. Alone action of Ins(1,4,5)P3 decreased common calcium content by 42,94±11,82 % (p=0,046, n=8), ryanodine – by 59,31±13,32 % (p=0,045, n=6), rutenium red – by 32,84±7,71 %. We observed increasing of membrane-bound Ca2+ at ryanodine presence in incubation medium by 28,5% (p=0,01, n=8), also Ins(1,4,5)P3 caused statistically authentic increasing of membrane-bound Ca2+ by 40% comparison to control. Simultaneous presence of ruthenium red (10-5M) and ryanodine (5nM) in incubatium medium did not cause any statistically significant changes of membrane-bound Ca2+. In contrast to simultaneous presence of ruthenium red (10-5 M) and Ins(1,4,5)P3 (10 µM) evoked statistically significant (p=0.0014, n=7) increasing of calcium content in comparison with control medium by 34,74±12.90 %. We made conclude, that are difference relations between mitochondria and RyRs and between mitochondria and IP3Rs in salivary glands secretory cells of Chironomus plumosus larvae L.