Introduction: Traditional (concentric) resistance training has been shown to reduce falls risk, and increase strength, mobility and cognition in an older adult population. Eccentric exercise is superior to traditional training at improving strength in young participants and may, therefore, provide a more effective training stimulus to improve function in the older adult. We carried out a feasibility study to investigating the effectiveness of a 4-week eccentric exercise protocol to improve strength, frailty, mobility and cognition in a group of older (>65 yr) participants. Methods: Six older (>65 yr) participants (mean±SD; 70 ± 5 yr) who had a low/medium cardiovascular risk score, no active associated medical conditions and could mobilise independently took part in the study. Each participant completed eight, 15-minute sessions of eccentric stepping exercise (EccentronTM, BTE, Hanover, MD, USA), over four weeks, with intensity matched to ‘hard’ (i.e. 15 on the Borg perceived exertion scale). The following assessments were completed before training, shortly after and at 30-day follow-up: maximal voluntary contractions (MVC) to assess eccentric, concentric and isometric strength, the frailty phenotype, the Montreal cognitive assessment (MoCA) and the elderly mobility scale (EMS). Results: Following 4 weeks of eccentric training, eccentric, concentric and isometric strength increased by 37.6 ± 23.0% (P<0.05), 25.6 ± 24.1% (P<0.05) and 21.4 ± 19.8% (P=0.063), respectively. There was no significant difference between post-training strength and 30-day follow-up for eccentric (39.8 ± 23.6%, P=0.176), concentric (28.4 ± 27.9%, P=0.735) or isometric (25.8±12.3%, P=0.310) strengths. No cognitive impairment was recorded at any point. There was no difference in frailty phenotype activity levels (P=0.345) or MoCA (P=0.335) following eccentric training. The EMS functional reach increased in 5 participants resulting in a 22.6 ± 24.5% increase from baseline (29.3 ± 7.1 vs. 34.9 ± 6.7 cm), but this did not reach significance (P=0.115). Conclusion: A short eccentric exercise programme can increase muscle strength in older adults, with these benefits retained at 30-day follow-up. Eccentric stepping provides an effective training stimulus for elderly subjects in an easy to use and safe manner, with benefits retained post-intervention. However, training elderly subjects with a single modality of exercise may not address all aspects of strength and daily function.