Background: Dietary supplementation with inorganic nitrate (NO3-) has been reported to lower blood pressure (BP) in healthy adults. This effect has been attributed to an increase in circulating plasma nitrite (NO2-) and subsequent nitric oxide (NO) synthase-independent NO generation. Cold exposure increases vascular resistance and BP, effects which are partly mediated by an attenuation in NO synthase-derived NO. Since cold exposure also elevates salivary flow rate, which would be expected to increase NO3- secretion by the salivary glands for reduction to NO2- by oral bacteria, this study tested the hypothesis that acute dietary NO3- supplementation would increase salivary and plasma [NO2-] and lower BP to a greater extent in cold compared to normothermic conditions. Methods: Twelve healthy males volunteered to participate in this study. Participants reported to the laboratory on four occasions for assessment of oral and mean skin temperature, salivary flow rate, salivary and plasma [NO3-] and [NO2-], BP and resting skin perfusion. These measurements were completed in an environmental chamber at baseline, with the temperature fixed at 28°C. Subsequently, participants ingested 140 mL of concentrated NO3–rich (BR; 8.4 mmol NO3-) or NO3–depleted (PL) beetroot juice. Measurements were then repeated over 3 h at either 28°C (normothermia) or 20°C (cold). The four experimental trials, BR and PL ingestion in normothermic (BR-Norm and PL-Norm) and cold (BR-Cold and PL-Cold) conditions were administered in a double-blind, repeated-measures, cross-over experimental design. Data were analysed using ANOVAs and paired t-tests. Values are presented as means ± SD. Results: Oral and mean skin temperature were lower throughout the cold conditions compared to the normothermic trials (P<0.05). Salivary flow rate was greatest in the BR-Cold and PL-Cold conditions (P<0.05). Salivary and plasma [NO2-] were greater post BR ingestion in the BR-Cold compared to the BR-Norm condition (P≤0.06). Systolic BP was lower at 3 h in BR-Norm (113 ± 10 mmHg) compared to PL-Norm (117 ± 6 mmHg; P<0.05). Systolic BP increased above baseline in the cold conditions, with the mean increase being lower in BR-Cold (3 ± 6 mmHg) compared to PL-Cold (7 ± 7 mmHg; P<0.05). There was no difference in skin perfusion between BR-Cold and PL-Cold (P>0.05). Conclusions: These results suggest that consumption of NO3–rich beetroot juice is more effective at increasing salivary and plasma [NO2-] and lowering systolic blood pressure in cold compared to normothermic conditions. Importantly, NO3–rich beetroot juice does not alter skin perfusion or compromise thermoregulation in the cold. These findings might have implications for attenuating the cardiovascular strain that accompanies cold exposure.