Creatine (Cr) supplementation during resistance training (RT) increases muscle mass and strength. However, it is unknown whether the volume and frequency of Cr ingestion will influence the adaptations from RT. This study determined the effects of equal-volume Cr supplementation with different dosing frequency on muscle hypertrophy and strength. Young healthy adults (N=22; 11 male, 11 female; 21±3 years) were randomized to supplement with Cr during 2 days/week of RT (Cr2, 0.15g/kg-1, N=11, 6 male, 5 female) or 3 days/week of RT (Cr3, 0.10g/kg-1, N=11, 6 male, 5 female). Creatine was consumed (in equal amounts) immediately before and immediately after each RT session. The RT program consisted of 2 sets (Cr2) or 3 sets (Cr3) of 10 repetitions (10RM) to muscle fatigue for 9 whole-body exercises. Prior to and following Cr supplementation and RT, measurements were taken for muscle hypertrophy (elbow and knee flexors and extensors; ultrasound), and muscle strength (1-repetition maximum leg press and chest press). Repeated measures ANOVA (mean ± SD) showed creatine supplementation increased muscle hypertrophy and strength over time (p<0.05). There was a greater change in leg press strength in the Cr2 group (Cr2: 223±93→362±220kg, 62% vs. Cr3: 168±54→238±112kg, 42%, p=0.05). There were no differences between groups for changes in muscle size of the elbow flexors (Cr2: 2.9±0.9cm→3.2±1.0cm, 10% ; Cr3: 2.7±0.6→3.0±0.6cm, 11%), elbow extensors (Cr2: 3.4±1.2→4.3±0.7cm, 26%; Cr3: 3.0±1.2→3.7±0.9cm, 23%), knee flexors (Cr2: 5.0±1.0→5.4±0.7cm, 8%; Cr3: 4.7±1.1→5.4±0.8cm, 15%), and knee extensors (Cr2: 4.3±1.0→4.7±1.4cm, 9%; Cr3: 3.7±0.7→3.9±0.7cm, 5%), or chest press strength (Cr2: 109±66→128±73kg, 17%; Cr3: 77±49→85±42kg, 10%). These results suggest that the volume of Cr ingestion may be more important than the frequency of Cr ingestion for improving lower-body muscle strength in young healthy adults.