Numerous evidence demonstrates the antinociceptive effect of the magnetic field. However, the analgesic action mechanism of the electromagnetic field (EMF) is not exactly understood. The aim of present study was to investigate the effects of 5-HT1 and 5-HT2 receptor agonists (serotonin HCl and DOI hydrochloride) on EMF induced analgesia. In total, 66 adult male Wistar albino rats with an average weight of 225±13 g were used in this study. The animals were subjected to repeated exposures of alternating 50 Hz and 5 milli Tesla (mT) EMF for 15 days. Prior to analgesia tests, serotonin HCl (5-HT1 agonist) 4 mg/kg, WAY 100635 (5-HT1 antagonist) 0.04 mg/kg, DOI hydrochloride (5-HT2 receptor agonist) 4 mg/kg and SB 204741 (5-HT2 antagonist) 0.5 mg/kg doses were injected into rats. For statistical analysis of the data, analysis of variance (ANOVA) was used and the multiple comparisons determined by Tukey test. Administration of serotonin HCl magnetic field (5 mT) exposed rats produce a significant increase in % MPE as compared to EMF group (p<0.05). On the contrary, injection of WAY 100635 to magnetic field exposed rats produce a significant decrease in analgesic activity (p<0.05). Similarly, administration DOI hydrochloride significantly increases % MPE values as compared to EMF group while SB 204741 reduces it (p<0.05). In conclusion, our results suggested that serotonin 5-HT1 and 5HT2 receptors play an important role in EMF induced analgesia. However, further research studies are necessary for the mechanism of action of the magnetic field.