Human populations exhibit temporal and cross-sectional variations in fatty acid intake. Variations in dietary saturated (butter, SFA), polyunsaturated (PUFA) and trans fatty acid (TFA) intakes altered cardiovascular disease risk in humans1. In rats, maternal diets with high levels of fat induce vascular dysfunction2, while maternal omega-3 PUFA deficiency is also associated with hypertension in adult offspring3. However, to date there is no information about the effect of maternal diets with sub-optimal total fat content on vascular function in the offspring. Female rats were fed either low (LF, 3.5% w/w) or adequate (AF, 7% w/w) safflower oil (SAO, enriched in linoleic acid), hydrogenated soybean oil (HSO, enriched in TFA), butter (enriched in SFA) or fish oil (FO, enriched in eicosapentaenoic and docosahexaenoic acids) from two weeks prior to mating until offspring were weaned at day 28 onto AIN93M (4% soybean oil w/w). Vascular constriction to phenylephrine (Pe 1nM to 100µM) and endothelium-dependent relaxation to acetylcholine (ACh 0.1nM to 10µM) were measured ex vivo in thoracic aortae of 70 day old offspring by wire myography (n=6 per group). Statistical analysis was performed on pEC50 and % maximum responses using 2-way ANOVA with Tukey’s post hoc test. There were no significant differences by amount or type of maternal total dietary fat in constriction of offspring aortae to Pe. There was a significant interaction (p = 0.002) effect of amount and type of fat on relaxation of female aortae to ACh, but there were no individual group differences in males or females. These data show that low maternal dietary fat does not affect aortic constriction to phenylephrine, nor does it affect endothelium-dependent relaxation. These data suggest that, unlike maternal high fat diets, maternal low fat diets do not induce vascular dysfunction in offspring. The findings, if confirmed in humans, would inform dietary advice offered to pregnant mothers.