Endurance training with ischemic preconditioning improves hematological profile and performance in distance runners

The Biomedical Basis of Elite Performance 2024 (University of Nottingham, UK) (2024) Proc Physiol Soc 62, C06

Oral Communications: Endurance training with ischemic preconditioning improves hematological profile and performance in distance runners

Ioannis Loukas1, Maria Koskolou1, Nickos Geladas1

1Department of physical education and sports science, Athens Greece

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Intro: Maximizing physiological adaptations is the key for optimal performance. Ischemic preconditioning is well-known to improve acute exercise capacity and performance. Aims/Objectives: The purpose of this study was to examine whether endurance training with ischemic preconditioning would enhance work of training and thus fortify physiological adaptations and increase performance.

Methods: Sixteen male distance runners (age: 34.1±5.1 yrs, weight: 70.8±4.1 kg) participated in the study. Training consisted of 2 high-intensity interval sessions (90-100% VO2max) and 3 continuous sessions (70-80% VO2max) per week for 2 months. Participants were divided into two groups of equal fitness level. Before interval training the ischemic preconditioning group (ISC, n=8) underwent 5 min total blood flow occlusion of each leg separately 3 times per session with pressure cuffs (250mmHg), while the control group (CON, n=8) underwent the same protocol but without pressure being applied. Pre and post training measurements of VO2max, hematological and cardiovascular variables were performed, while a field-specific test of 5x1000m with 2 minutes recovery was executed.

Results: Weekly running volume was similar between groups (ISC: 80.2±3 km vs CON: 80.6±4 km). ISC acheived faster (p<0.05) interval training average speed (18±0.4 km/h) than CON (17.4±0.3 km/h). Training increased VO2max (p<0.01) in both groups (ISC: 55.3±1.2 vs 60.1±1.8 ml/kg/min, CON: 55±2 vs 57.6±1.9 ml/kg/min) but the increase was higher in ISC (training x group interaction: p<0,05). Maximal aerobic speed (vVO2max) was also increased in both groups (p<0.01) (ISC: 18.4±0.9 vs 19.6±0.9 km/h, CON:18.2±0.7 vs 18.9±0.6 km/h) with a higher increase in ISC (training x group interaction: p<0.05). Post training running times during the 5x1000m test were faster in ISC than CON (p<0.05). ISC compared to CON showed a more pronounced increase in blood volume (5415±438 vs. 5103±517 ml) and plasma volume (3114±271 vs 2912±246 ml) (training x group interaction: p<0.05). Moreover, there was a training effect (p<0.05) on cardiac output, stroke volume, mass of hemoglobin and red cell volume without differences between groups.

Conclusions: Muscular ischemic preconditiong applied prior to a regular training mode may enhance physiological adaptations mainly through hematological alterations and, thus, improve athletic performance.

Ethical standards: The study was approved by the University’s Ethical Committee for human experimentation and conformed to the Declaration of Helsinki.



Where applicable, experiments conform with Society ethical requirements.

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