Phytoestrogens, the estrogen-like substances found in many plants, are being explored as an alternative to steroids for the treatment of estrogen-related disorders including cardiovascular diseases, menopausal symptoms, postmenopausal osteoporosis, etc. Cuminum cyminum (CC) is a phytoestrogen-rich plant. Fruits of CC are reported to contain estrogenic compounds like β-sitosterol, stigmasterol and luteoline. The present study investigated anti-osteoporotic potential of methanolic extract of fruits of CC (MCC) against postmenopausal osteoporosis in rats. Ovariectomized (OVX) Sprague Dawley rats were used as an experimental model for postmenopausal osteoporosis. Ovariectomy was performed under ketamine (80 mg/kg, i.p.) and xylazine (10 mg/kg, i.p.) anesthesia. MCC (1000 mg/kg, in two divided doses) and vehicle (0.5% CMC) were administered orally to OVX animals and Sham control/OVX control animals, respectively, for 10 weeks. Estradiol (0.15 mg/kg) was used as the standard for comparison. At the end of 10 weeks blood (for estimation of calcium (Ca), phosphorous (P), alkaline phosphatase (ALP) and tartarate-resistant acid phosphatase (TRAP)); urine for estimation of Ca, bones (for bone density, ash density, Ca content and mechanical strength) and uteri were collected. Students’ t test at 95% CI was used for statistical analysis. Treatment with MCC caused decrease in bone turnover as indicated by the significant decrease in serum ALP with concurrent decrease in serum TRAP. MCC did not alter calcium and phosphorous homeostasis which was seen by no effect on serum Ca and P as compared to Sham control and OVX control. MCC caused significant decrease in urinary excretion of Ca as compared to OVX control. There was increase in bone and ash density, significant increase in Ca content and Ca:P ratio of bone and significant increase in mechanical strength of the bone as revealed by three point bending test in tibia and femoral neck strength test. Serum total cholesterol was measured to assess the effect of MCC on increased cholesterol levels due to ovariectomy. MCC treatment did not cause any increase in uterine weight, uterine peroxidase (UPO) activity and total protein content of uterus. The results of the study underline the anti-osteoporotic potential of MCC in postmenopausal osteoporosis with additional benefit of cholesterol lowering capacity.