Cutaneous microcirculation reactivity can be quantitatively described by topical drug application and Laser Doppler Flowmetry (LDF). Nicotinates are often used for this purpose as vasodilator drugs, and the influence of age, gender and skin location on this response have been addressed. This work focuses the effect of local perfusion conditions on that response. 14 young healthy volunteers (22.7±2.8 years old) participated in this study, after giving their written informed consent. Methyl nicotinate (MN) was applied on the dorsum of a randomly chosen foot on two different circulatory dynamics protocols: (a) on the seated position while breathing room atmosphere and then breathing a saturated oxygen atmosphere; (b) on the supine position with both feet at heart position and while performing a passive leg raising (PLR, 45°). The microcirculation response was evaluated with LDF (PF 5010 system, Perimed, Sweden) for 15 minutes after MN application. Several curve-dependent parameters, including full response mean perfusion value and area under the curve (AUC) were compared for each protocol. Furthermore, the signal was assessed at a 32 Hz sampling frequency and analyzed with: the wavelet transform, giving its major components (cardiac, respiratory, myogenic, sympathetic, and endothelial); and the detrended fluctuation analysis (DFA), which allowed the characterization of the self-similarity properties through its alpha (α) exponent. The first 5 minutes of each volunteer’s plateau phase were used for comparison. All statistical comparisons were done with the Wilcoxon signed-rank test (p<0.05). MN induced vasodilation, steadily increasing perfusion, which was maintained on a plateau until reduction by dermal absorption. The saturated oxygen breathing, a constrictive stimulus, did not significantly change the vasodilation parameters’ values. While breathing the saturated oxygen atmosphere, a significantly lower myogenic activity was noted (p=0.006). No changes for the cardiac, respiratory, sympathetic or endothelial activities were detected. Although no difference as found for the respiratory activity, this alpha exponent was found to be significantly higher while breathing a saturated oxygen atmosphere (p=0.038). The PLR technique reduced the magnitude of vasodilation significantly by reducing the mean perfusion level and AUC. Significant lower activity and alpha exponents were noted for the cardiac component (p=0.004 and 0.005, respectively) relative to the supine position. No differences were found for the activities and alpha exponents of the other components. These results suggest the usefulness of this in vivo model, as well as the potential of the wavelet transform and DFA for the functional analysis of the peripheral vascular activity.