Background: Regular diet, balanced nutrition and adequate rest are very important to achieve fitness and therefore sportspersons and athletes are very particular about these three things in their daily routine. For Muslims, the major religious period of the Islamic calendar is Ramadan when they undergo fasting from sunrise to sunset. This leads to irregularity in sleep and dietary patterns of fasting individuals during this holy fasting month. Objectives: The present study was aimed to explore the effect of Ramadan intermittent fasting (RIF) on fitness profile in sedentary male Muslims of Kolkata, India.Materials and Methods: Fifty (50) sedentary healthy male Muslims (age range 20-30 years) who were religiously participating in the RIF were randomly sampled for the study from Kolkata. Their personal demographic data, health status and consent to participate in the study were obtained through questionnaire. The mean age, body height and body mass were 27.17±5.29 years, 165.13±7.14 cm and 57.91±9.20 kg respectively. Their agility (shuttle run test), flexibility (sit and reach test), jump height (Astride jump test), hand grip strength, aerobic and anaerobic capacities and pulmonary function tests were measured in each subject on 7 occasions – 15 days before RIF, 1st day of RIF, 7th day of RIF, 15th day of RIF, 21st day of RIF, last day of RIF and 15 days after RIF. Selective blood parameters, (e.g., total count, differential count, erythrocyte sedimentation rate or ESR and haemoglobin percentage) were measured before and after RIF. During all the visits, subjects reported in the laboratory at 8AM and the parameters were measured thereafter.Results and Conclusion: Values of the studied parameters were within the normal physiological limit. Analysis of data by repeated measure ANOVA depicted insignificant change in the in all the studied parameters during the Ramadan. However, analysis of data by t-test revealed significant main effect for time on aerobic power, anaerobic power and agility which thus depicted significant occasional variation during the month of Ramadan. The values of RBC Count, WBC Count, Haemoglobin concentration, ESR during the first hour and second hour before and after the month of Ramadan did not show any significant variation. It is therefore concluded from the study that RIF did not impose any significant variation in fitness profile in general, but occasional variation in some of the parameters has been noted during the month of Ramadan in the studied population.