Regenerative medicine, nanomedicine, and tissue engineering have progressed for the developing of new techniques and methods of analysis to study tissue regeneration and biological functions of organs and tissues. We investigated whether myography was a method of analysis to assess function of the rectus abdominis muscle (RAM). Methods: The project was developed according to the animal experimentation rules CONCEA, approved by the Ethics Committee of Botucatu Medical School (number 1234/2017-CEUA) Seven virgin young female rats (n=7), aged ~60 days (286 ± 14 g), were used to harvest the RAM after euthanasia by decapitation. RAM sections of ~1.5 cm were collected from the pubis bone towards the central portion and immersed in Krebs solution at 8ºC (mM: NaCl 118.5, KCl 4.7, NaHCO3 25, 1.2 MgSO4, 1.2 KH2PO4, 2.5 CaCl2, 5.5 D-glucose, pH 7.4). RAM sections were mounted in the bath chamber of a myograph (Model 820MS, Danish Myo Technology) containing Krebs solution (37ºC) with constant bubbling (10% CO2, 90% O2). After 1 hour stabilization the response of RAM to electrical stimulation (20 Volts, 2 Hz, 1 ms pulse width, 500 ms pulse interval, bipolar pulse shape for 1 h:6 min:35 sec). Results: RAM responded to 85% ± 8 (p ≤0,01, Tukey’s post hoc test) of the applied pulses. An average of 19.4 ± 1.7(p ≤0,01,) mN (right RAM) and 18.7 ± 2.6 (p ≤0,01,) mN (left RAM), an average for maximum contraction force was obtained and loss of strength (fatigue) was verified at the 52.7 ± 11.7 (p ≤0,01,) min. Conclusion: Myography proved to be useful in analyzing RAM functionality. The muscle functionality (muscle contraction strength, fatigue and response to electrical stimulation) of RAM of young virgin rats could be properly assessed. The standardization of the myography analysis unprecedented in the literature has been successful. This approach may be useful in the characterization of muscle diseases and disorders as well as new therapies development.
Future Physiology 2021 (Virutal) (2021) Proc Physiol Soc 47, PC62
Poster Communications: Functional ex vivo analysis by myography of the female rat rectus abdominis muscle
Juliana Ferreira Floriano1, 2, 3, SOFIA VEGA QUIROZ1, 2, 4, Rafael Guilen de Oliveira1, 2, Angelica M.P. Barbosa1, 2, 5, Marilza V.C. Rudge1, 2, Luis Sobrevia2, 4, 6, 7, 8
1 Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo., Brazil 2 Medical School (Faculty of Medicine), Sao Paulo State University (UNESP), Brazil, Botucatu, São Paulo., Brazil 3 National Heart and Lung Institute, Imperial College London, London, United Kingdom 4 Cellular and Molecular Physiology Laboratory (CMPL), Department of Obstetrics, Division of Obstetrics and Gynaecology, School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile 5 Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences, São Paulo State University (UNESP), Marilia, Brazil, Marilia, São Paulo., Brazil 6 Department of Physiology, Faculty of Pharmacy, Universidad de Sevilla, Sevilla, Spain 7 University of Queensland Centre for Clinical Research (UQCCR), Faculty of Medicine and Biomedical Sciences, University of Queensland, Queensland, Australia 8 Department of Pathology and Medical Biology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands
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Where applicable, experiments conform with Society ethical requirements.