Diabetes mellitus impairs small intestinal motility; however, little studies have demonstrated the effect of garlic oil on small intestinal motility, so .his study evaluated the beneficial effects of garlic oil in type 2 diabetes mellitus. Thirty-six adult female Wistar rats were allocated into: control (C), garlic oil supplemented non-diabetic, diabetic, and garlic oil-treated diabetic groups. Rats were anesthetized with pentobarbitone (40 mg/kg BW), then small intestinal segments were studied for motility parameters and oxidative markers. Nasoanal length, waist circumference, fasting blood glucose level (FBG) and plasma insulin level were determined. Compared to control group, diabetic rats had reduced average force of contraction and motility index in all small intestinal segments, accompanied by decreased average duration of contraction only in jejunum, in addition to hyperglycemia, insulin resistance, prominent oxidative stress and obesity denoted by motility parameters, fasting blood glucose, HOMA-IR, intestinal MDA and waist circumference. Garlic Oil non-diabetic rats had reduced average force of contraction and motility index in all small intestinal segments, despite persistent higher Lee index and waist circumference. However, Garlic oil treated-diabetic rats had improved effects on small intestinal motility in almost all small intestinal segments and controlled the oxidative stress. In conclusion, decreased small intestinal motility was present in DM, mostly by oxidative stress, and in normal rats supplemented with garlic oil. However, garlic oil treatment in diabetic rats resulted in an improvement in small intestinal motility and in a remarkable anti-hyperglycemic effect, mostly due to its antioxidant effect.