Exercise is associated with changes in brain activity. Studies have examined few EEG frequency bands using few electrodes. There are few reports on EEG activity in response to acute exercise between male and female. We investigated how EEG frequency bands change in females in response to acute exercise compared to males. Consenting healthy adult females (n=15) & males (n=15) bicycled an automated ergometer at 50% HRmax for 20 min. A 16-channel EEG was recorded using international standards from mid-frontal (F4 & F3), central (C4 & C3), parietal (P4 & P3), temporal (T4 & T3) & occipital (O2 & O1). Exercise-induced EEG changes were compared between two sexes by Mann Whitney test. EEG power (μV2) is presented as median & interquartile range. The study was approved by institute ethical committee. In female, as compared to male, resting right side delta, alpha, beta & gamma activities were more in almost all recorded sites [delta: F4=49.82(44.23-63.56) vs. 35.5(32.70-44.44), p<0.001; C4=48.79(38.34-58.64) vs.37.6(26.72-43.80), p<0.01; P4=48.6(43.41-53.12) vs. 35.08(29.48-38.98), p<0.001; O2=58.88(52.65-59.52) vs.34.6(28.16-47.80), p<0.001; T4=17.78(15.77-20.95) vs.14.13(11.74-17.34), p<0.05], [alpha F4: 127.62(112.89-149.03) vs. 49.36(46.37-52.98), p<0.001; C4: 124.95(111.95-147.25) vs.57.07(51 – 59.29), p<0.001; P4=177.42(111.03-196.64) vs.89.91(66.58-98.86), p<0.001; etc], [beta F4=18.96(15.83-25.38) vs.14.77(10.34-17.55), p<0.05; C4=21.16(18.4-25.9) vs.15.48(9.66-19.40), p<0.01; P4=22.88(18.97-32.84) vs.18.7(12.32-24.09), p<0.05; etc], [gamma F4=0.58(0.44-0.87) vs. 0.24(0.20-0.42), p<0.001; C4=0.52(0.36-0.7) vs. 0.22(0.20-0.40), p<0.01; P4=0.54(0.45-0.67) vs. 0.24(0.21-0.40), p<0.001; etc]. Similarly, female resting right theta activity was more in Parietal [P4=33.04(25.1-42.41) vs.22.3 (18.36-34.33), p<0.05] & occipital [O2=50.81(30.64-66.8) vs.26.85 (22.18-34.42), p<0.001] regions than in male. They had similar picture on the left side also. The delta values (difference between post exercise and resting) of right alpha power was less in female in frontal [F4= -11.61(-45.24 -3.64) vs.9.48(1.05-16.58), p<0.01] and central [C4= -72(-32.98-9.48) vs.22.69(13.03-33.05), p<0.01] regions compared to males but they had less left central alpha [C3= -8.32(-32.65-6.1) vs.16.5(0.36-36.36), p<0.01] and temporal beta [T3= -6.29(-10.09- -1.49) vs.1.24(-0.84- 2.8), p<0.001] power compared to male. At rest, female group had more power of all the EEG frequency bands than the male. After acute exercise the female had decreased alpha in right frontocentral region whereas left alpha activity was reduced in central region and beta activity in temporal region. Although at rest females may have high EEG powers in different bands, the actual change in response to exercise, they respond in reverse way as compared to males. They seem less relaxed than the males immediately after moderate intensity of exercise.