In recent years, it is becoming increasingly apparent that insulin signaling regulates neural circuits in the brain, playing roles in the control of appetite, cognition and memory. However, the detailed action of insulin in the brain has not yet been fully explained, especially in respect of food intake. Here, firstly we performed intranasal FITC-insulin to track the distribution of insulin receptor in the mice brain to highlight the areas of the brain that intranasal delivery can reach. In addition, monitored the metabolic changes after delivering intranasal Standard-insulin in mice. Our results show that the insulin receptors distribute many areas of mice brain, including olfactory bulb, nucleus of the horizontal limb of the diagonal band, hippocampus, and brainstem. And the intranasal insulin could reduce food consumed and oxygen consumption, and ambulatory activity. Overall, our data indicates that intranasal delivery of insulin is a good way to target areas of the brain important for controlling energy balance. Due to the metabolic effect that intranasal insulin have both in rodents and humans, further studies are warrant in order to understand the which insulin sensitive neurons are involved in this process.