Increased memory and decreased naive T cells in human hypertension

Physiology 2014 (London, UK) (2014) Proc Physiol Soc 31, PCA128

Poster Communications: Increased memory and decreased naive T cells in human hypertension

P. J. Marvar1,2, T. D. Cruise1, E. C. Hart1,3, A. Burchell1,3, L. Ratcliffe1,3, A. K. Nightingale1,3, J. F. Paton1,3

1. Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom. 2. Pharmacology and Physiology, George Washington University, Washington, District of Columbia, United States. 3. CardioNomics, School of Physiology and Pharmacology, University Hospitals Bristol, Bristol, United Kingdom.

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Studies have revealed a role for pro-inflammatory effector-like CD3+ T cells in the pathogenesis of experimental hypertension (Guzik et al., 2007). Despite these observations, our knowledge of the role that T cells and subsets of T cells (memory vs naïve) play in the development of human hypertension is unknown. We hypothesized that circulating cells of hypertensive patients exhibit a pro-inflammatory T cell phenotype, as determined by the balance of memory (effector) versus naïve CD3+ cells. Using flow cytometry, subsets of circulating T cells and markers of antigenic memory were analyzed in male hypertensive patients and normotensive subjects. There were no differences in the overall percentage of CD3+ cells in normotensive (63.3 ± 3.7%) (n=5; mean age 34 yrs; mean systolic BP 128.6 mmHg) vs. hypertensive men (62.2 ± 5.5%) (n=6; mean age 53.5 yrs; mean systolic BP 154.7 mmHg). The percentage of CD4+ and CD8+ T cell subsets were not statistically different between groups. Further subset analysis revealed that the ratio (1.6 ± 0.3 vs 0.7 ± 0.1; P <0.05) of memory (CD45RO+CD3+) vs. naïve (CD45RA+CD3+) T cells as well as the percentage of CD8+CD62L(low) cells (70.4 ± 4.5 vs 52.8 ± 6.3; P <0.05) were increased in hypertension. These results demonstrate an imbalance in effector memory / naïve T cells in hypertensive men and provide further evidence that chronic antigen exposure may be involved in the pathogenesis of hypertension.



Where applicable, experiments conform with Society ethical requirements.

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