Insight into the mechanism of D-glucose accelerated exchange in GLUT1 from molecular dynamics simulations

Physiology 2023 (Harrogate, UK) (2023) Proc Physiol Soc 54, PCB038

Poster Communications: Insight into the mechanism of D-glucose accelerated exchange in GLUT1 from molecular dynamics simulations

Richard Naftalin1, Saul Gonzalez-Resines1, Carmen Domene1,

11Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK2 Bath United Kingdom, 21Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK2 Bath United Kingdom, 3BHF Centre of Research Excellence, School of Medicine and Life Sciences, King’s College London London United Kingdom,

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Atomistic molecular dynamics simulations demonstrate that when multiple β-D-glucose molecules are present within the GLUT1 transporter, simultaneous position exchanges frequently occur between adjacent ligands. These exchanges take place in the internal cavities and at both external and internal solution interfaces of the protein. They involve rotation of adjacent ligand positions along the central pore axis of the transporter with variable duration in the nanoscale (4 – 100 ns). Exchanges occurring at the extracellular protein interfaces involve fast displacements (2 – 10 ns) of D-glucose  H-bonded to the protein interface by other D-glucose  molecules present in solution.  These examples of simultaneous D-glucose  exchanges demonstrate that accelerated exchange is consistent with a multisite model for D-glucose  transport within GLUTs where multiple D-glucose  molecules move independently and stochastically within the transporter’s tunnels, cavities, and the central pore.

Higher frequency of D-glucose exchange is observed in the membrane gel state, corresponding with D-glucose transport in human erythrocytes at low temperatures. The presence of multiple D-glucose  molecules both within the transporter and in bathing solutions increases D-glucose  penetration depths from the solutions into transporter intramembranous zones, particularly in the gel state.

That exchange frequency between adjacent ligands depends on the local D-glucose  density within the transport pathway explains why accelerated exchange occurs more frequently in conditions where bottlenecks at the openings of the transport pathway are prolonged, at low temperatures (1), thereby augmenting ligand aggregation in the adjacent upstream regions.



Where applicable, experiments conform with Society ethical requirements.

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