High blood glucose has been linked with increased glucose concentration in the airway surface liquid. This has been shown to be associated with an increased risk of developing a pulmonary infection in patients in intensive care unit and in patients who have diabetes and chronic lung disease 1. Contrary to popular belief that lungs are sterile, it was recently discovered that lungs are inhabited by resident bacteria. Several chronic lung conditions, such as cystic fibrosis (CF) and chronic obstructive pulmonary disease, have been shown to induce changes to the lung bacteria. To investigate the effects of hyperglycaemia on the pathogens residing in the lungs of CF patients, we have used Artificial Sputum Medium (ASM) 2. ASM was designed to imitate sputum found in CF patients. Staphylococcus aureus (ATCC29213) and Pseudomonas aeruginosa (PAO1) were used for experimental design as they are some of the most common CF pathogens. S. aureus and P. aeruginosa were grown in the ASM at 0mM and 8mM glucose. Approximately 3x107bacteria were added to a 50ml tube. Bacteria were then grown at 37°C with constant shaking. Growth curves were constructed by quantifying CFUs using serial dilutions. Interestingly when performing colony counts for the CFU calculations, it was impossible to count individual colonies of P. aeruginosa. When P. aeruginosa was grown in both ASM and Mueller-Hinton (MH) medium in parallel, colonies from MH medium formed as expected, but formed a single spread super-colony when plated from ASM. As colony counting was not possible, bioluminescent P. aeruginosa (H174) was used to construct the growth curve instead. With this strain, production of light was used as an indicator of viable, metabolically active cells. In the presence of 8mM glucose, S. aureus demonstrated increased growth rate and increased peak bacterial population when compared to no glucose. Glucose did not have the same effect on the growth of P. aeruginosa as it did on the S. aureus. In presence of glucose, P. aeruginosa grew slower than in the glucose-free environment. Despite the slower growth rate, the peak population and total bacterial population were higher in the presence of 8mM glucose. When P. aeruginosa wasplaced into the ASM the population decreased by 90% within the first ten hours. Afterwards it increased by 100-fold from the original population. The decrease in growth was observed repeatedly with varying initial populations of P. aeruginosa. In conclusion, ASM mimics the nutrients available in the lungs and allows the studyof lung pathogen growth patterns in the presence of glucose. We speculate that initially P. aeruginosa utilises mucin and amino acids from ASM for growth and begins to use glucose when other nutrient sources are depleted. The possibility of co-culturing of multiple pathogens could unveil complex growth interactions between bacterial species residing within the lungs.