Known as a multifactorial disease, prostate cancer (PCa) is considered one of the most frequently diagnosed tumors and second leading cause of cancer related deaths among men. We aimed to evaluate the relationship between manganese superoxide dismutase (MnSOD) Ile-58Thr, catalase (CAT) C-262T and myeloperoxidase (MPO) G-463A gene polymorphisms and the susceptibility and clinicopathological characteristics of PCa. One hundred and fifty five patients diagnosed with PCa and 195 controls with negative digital rectal examination and PSA<4ng/dl were enrolled in this study. MnSOD, CAT and MPO gene polymorphisms were performed by polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP) methods. For the statistical analyses Pearsons’s Chi-Squared (χ2), Mann-Whitney U and multiple logistic regression model tests were used where appropriate. No statistically significance regarding demographic data and smoking status was observed between groups. As expected, total PSA levels were significantly elevated in the PCa patients compared with controls. No relationship was found between MnSOD Ile-58Thr gene polymorphism and PCa risk. However, there were significant differences in the genotype distributions of CAT C-262T and MPO G-463A gene polymorphisms between PCa patients and controls (aOR:1.57; 95%CI: 1.11-2.21; p=0.010; aOR:1.78; 95%CI: 1.15-2.76; p=0.009, respectively). MnSOD Ile-58Thr and MPO G-463A gene polymorphisms were not found to be associated with clinicopathological characteristics including pathological grade, T stage and metastasis among PCa patients. However, CAT C-262T gene polymorphism was found to be associated with clinicopathological characteristics including T stage and metastasis among PCa patients. The patients with PCa carrying TT genotype had a significantly increased risk of high pathological T stage disease and metastasis compared to patients carrying CC genotype (aOR:1.94; 95%CI: 1.14-3.23; p=0.014; aOR:3.83; 95%CI: 1.75-6.59; p=0.000, respectively). It seems that there is no association of PCa and MnSOD Ile58Thr polymorphism, whereas TT genotype in CAT C-262T polymorphism and GG genotype in MPO G-463A polymorphism may be associated with increased PCa risk. The TT genotype in CAT C-262T polymorphism may also play as a risk factor in tumor progression and metastasis among Turkish men.