The carotid bodies, which detect changes in arterial PO2 and elicit a peripheral chemoreflex (PCR), are particularly important in their role in acclimatization to altitude. Ventilatory acclimatization increases the PCR magnitude at altitude, improving oxygenation, and potentially protecting against acute mountain sickness (AMS). The PCR magnitude can be tested through steady-state techniques that require sophisticated equipment and lack portability, decreasing their utility in field studies at altitude. The transient hypoxia test is simple and more portable, but making participants more hypoxic while at altitude may be uncomfortable and is potentially dangerous. We tested the feasibility of using a transient hyperoxic ventilatory withdrawal (TT-HVW) test of the PCR while in the setting of simulated high altitude (normobaric hypoxia), and compared these responses to (a) a previously characterized transient hypoxic ventilatory response (HVR) test (TT-HVR) in room air and (b) a poikilocapnic hypoxia test during acute steady-state hypoxia (SS-HVR), within-individuals. Participants were recruited (n=15; 28.5±6.5 yrs; BMI 24.7±3.1 kg/m2; six males; SD) and were positioned in a dentist chair in semi-recumbent position in a dark room with white noise fed through ear buds. Participants were instrumented with a pneumotachometer and end-tidal gases were sampled from the mouthpiece in percent using a dual O2 and CO2 gas analyzer, and expressed in mm Hg using dialing atmospheric pressure (BTPS). While breathing room air, participants underwent five consecutive trials of a TT-HVR (three-breaths of 100% N2) to calculate HVR magnitude. Participants were then exposed to a fraction of inspired (Fi)O2 of 13.5-14% (simulated 4500-5000m in Calgary; Patm ≈665 mm Hg) for 25-30 minutes until steady-state was achieved, where an additional index of the poikilocapnic HVR was calculated by comparing ventilation (L/min) and calculated oxygen saturation (ScO2) between baseline and steady-state hypoxia. Three consecutive trials of a TT-HVW (one-breath 100% O2) were then administered and quantified. The TT-HVR response (0.37±0.04 △L/min/△%ScO2) was larger than the TT-HVW (0.22±0.03 △L/min/△%ScO2; P<0.001) and the SS-HVR (0.1±0.01 △L/min/△%ScO2; P=0.003; all ±SEM; all one-f RM ANOVA). No test of the PCR was correlated with any other test (r<0.32, P>0.25), nor was any test of the PCR correlated with indices of oxygenation while in steady-state hypoxia: partial pressure of end-tidal (PET)O2 (r<0.2, P>0.47) or ScO2 (r<0.2, P>0.48; all Pearson r correlations). Our results demonstrate that any test of the PCR at low altitude does not predict indices of oxygenation while in steady-state hypoxia, and may have minimal utility in predicting ventilatory acclimatization and protection from hypoxia during gradual ascent to altitude, where individuals are variably susceptible to AMS.