Background: Cardiovascular disease (CVD) remains the leading cause of death globally. In many cases, a leading cause of morbidity and mortality in patients receiving chemotherapy has been cardio-toxicity. Anthracyclines are widely used as chemotherapeutic agent and is now part of standard therapeutic regimens for a variety of cancers. Anthracyclines’ clinical use however is limited by its induced cardio-toxicity. Although a variety of approaches to protect the heart against Anthracycline-induced cardio-toxicity is currently part of clinical practice but the mortality rate of such patients is still high. The search for plant derived anti-oxidant peptides is on the increase. Such alternatives may be safer than other antioxidant as they are from food sources and have been consumed over the years without side effects.The present study was thus designed to evaluate the cardioprotective effect of Moringa Oleifera cyclotide (MOC) in a rat model of doxorubicin (DOX) induced cardiomyopathy Method: MOC fraction was obtained by solvent extraction and peptide purification. Male adults albino Wister rats (48) weighing between 180-200 g were randomly divided into six groups (n=8). Animal experimentation lasted for 17 days. Group 1 animals served as control group and were untreated. Group 2-6 animals were induced with cardiomyopathy by Intra-peritoneal injection of DOX (5 mg/Kg) at day 15. Group 4 was pre-treated on day 15 with dexamethasone (3mg/kg) 20 minutes before DOX treatment. Group 3-6 were orally pre-treated with MOC 10, 20, 40 mg/kg respectively for 15 days. Cardiovascular measurements and animal sacrifice were done 48 hours after DOX treatment. plasma and tissue samples were obtained for biochemical, histological and molecular assays. Result: Rats treated with doxorubicin alone (Group 2) showed high LF/HF ratio, increased blood pressure, cardiac hypertrophy, disseminated congestion of cardiac tissue, raised homocysteine, cardiac troponin I and KIM-1 levels when compared with control. However, MOC pre-treatment especially at 10 mg/Kg ameliorated the adverse DOX induced cardiac dysfunction which was comparable with animals pre-treated with dexamethasone. Conclusion: MOC exhibits cardio-protective effects against doxorubicin induced cardiac dysfunction in male adult albino Wister rats.