Supine neural hemodynamic balance is present in healthy young, but not older men (Hart et al., 2009). However, humans spend two thirds of their time upright; extending these observations to the upright posture will be important to ascertain the impact of sympathetic activity in human hypertension. We compared the neural hemodynamic balance in healthy young and older men without any sign of hypertension (24 hour ambulatory blood pressure (BP) monitoring), comorbidities or classic cardiovascular risk factors. Cardiac output (Qc, C2H2 rebreathing), BP and muscle sympathetic nerve activity (MSNA) were measured in 10 healthy young and 10 healthy older men in the supine and passively tilted (30° and 60°) postures. By design, the two groups differed by age (Δ34 yrs, [26,41]). Supine Qc (Δ-3.8, [-4.7,-2.9] l·min-1), and systolic BP (Δ-12, [-23,-0.8] mmHg) were lower in the healthy older group. Mean arterial pressure tended to be lower (Δ-5.1, [-10,0.19] mmHg). Conversely, MSNA burst incidence (Δ23, [15,31] beats·100 hb−1) and total peripheral resistance (TPR, Δ,577 [419,734] dyne·s·cm−5) were increased in the healthy older group. Diastolic BP was similar in both groups (Δ-1.8, [-6.3,2.7] mmHg As shown previously, MSNA burst incidence was negatively related to Qc (R2=0.43, P=0.04) and positively related to TPR (R2=0.74, P=0.001) in the young group whilst supine. MSNA burst incidence was unrelated to Qc (R2=0.12, P=0.12) or TPR (R2=0.01, P=0.77) in the healthy older group whilst supine. In contrast to previous data, MSNA burst incidence was related to diastolic BP in the young (R2=0.57, P=0.01), but not the healthy older group (R2=0.15, P=0.26). In contrast to the supine posture, burst incidence in the young group was not statistically related to Qc or TPR in any steady-state upright posture (all P>0.05). Similar to the supine posture, MSNA burst incidence in the older group was not related to Qc or TPR in any upright posture (all P>0.05). In the young group, the relationship between MSNA burst incidence and diastolic BP became less pronounced after the acute transition into the 30° and 60° upright posture, but returned after 20 minutes at 60° upright (R2=0.51, P=0.02). Again similar to the supine condition, MSNA burst incidence in the older group was not related to diastolic BP in any upright posture (all P>0.05). Young men displayed neural hemodynamic balance in the supine posture, which was partially attenuated whilst upright. Neural hemodynamic balance was not apparent in the healthy older men in either posture. Interestingly, in this group of hemodynamically stable older men, MSNA was unrelated to BP in the steady-state condition. However, as Qc varied markedly between the older men and BP was normal, hemodynamic balance must have been maintained, at least in part, by non-neural mechanisms.