Skin is a complex biomaterial. One of its characteristics is anisotropy due to preferred fiber alignment in the skin, resulting from prolonged exposure to a surrounding stress state. Skin anisotropy is an important issue for plastic surgeons, as well as cosmetics science. In the last decades, several devices have emerged as indispensable tools in the study of the cutaneous physiology, as well as in the assessment of the efficacy and safety of topical formulations. The CutiScan CS 100® (CK electronics FRG) is designed to provide information not only about the elastic and viscoelastic properties but also on anisotropy and directionality of the skin. This device contains a probe that combines mechanical force with imaging in a unique way. It consists of a suction ring that draws the skin in all directions with a constant negative pressure. During the suction and recovery time a high resolution CCD camera inside the probe monitors the displacement of each pixel by an optical flow algorithm in a video. From that video an overall graph is generated, as well as interesting measurement parameters. The aim of this work is to conduct a systematic study of the opportunities and limitations of this equipment, as well as to compare its performance with that of other devices used to measure skin elasticity and anisotropy- the Cutometer® and the Reviscometer® (CK electronics FRG).20 volunteers participated in this study, after informed oral and written consent. Two groups were established, according to age. All procedures were conducted according to the principles of the World Medical Association’s Declaration of Helsinki. Measurements with the three devices were conducted in all volunteers in three different anatomical sites: forehead, volar forearm and lower leg. All measurements were conducted in triplicate. Additionally, in order to assess repeatability, ten consecutive measurements were made in the same site in the volar forearm. The parameters considered more relevant for analysis were Uf, Ua, Ur and Ue for the Cutometer, and those obtained at 0, 45, 90 and 135 degree angles for the Reviscometer and the CutiScan CS100.Results show significant correlations between the measurements obtained with the different devices, as well as with age of the volunteers. CutiScan CS100 and Reviscometer measurements were similar along the same axis. The devices performed differently in the repeatability test, however, all three provided satisfactory results. However, the CutiScan CS100 enabled a clearer and easier assessment of skin anisotropy, since it provides 360 degrees elasticity curves, that revealed the directions where the displacement and the returning rate were higher.In conclusion, the CutiScan CS100 can be employed in the study of the skin elastic and anisotropic behavior, in alternative to other methodologies that provide less detailed information.