Pregnancy and malaria are two aggrevating conditions resulting in increase in the production of reactive oxygen species. Studies had been conducted to investigate the antioxidant status of pregnant females with or without malaria parasitemia. Twenty four Plasmodium falciparum infected patients attending Adeoyo Maternity Hospital, Ibadan, Oyo State were compared with their age matched non-malarial pregnant women who served as control group. Antioxidant enzymes (catalase and superoxide dismutase), total antioxidant activity, nitric oxide as an index of oxidative status, some haematogical parameters and trace metals were measured. There were significant increases in catalase activities, selenium, iron and cadmium levels in the primigravidae malaria pregnant subjects as compared to their corresponding control group. Superoxide dismutase (SOD) activities and zinc levels are significantly decreased in malaria pregnant women in secundigravidae and multigravidae as compared to their corresponding control group. Zinc levels in the third trimester in the pregnant malaria subjects were lower as compared to their corresponding control group There were increased oxidative stress and concomitant antioxidant levels in malaria in pregnancy as compared to their corresponding control group. Also, there was significant positive correlation between nitric oxide level and catalase activities in pregnant malaria group (r = 0.918, P <0.01) The incidence of malaria in pregnancy is highest in nulliparous as compared to primiparous and multiparous women. It may be concluded that defense mechanisms against free radical damage are enhanced as pregnancy progresses and that maternal anemia as indicated in the low level of haemoglobin in pregnant malaria subjects remains the most frequent consequence of malaria during pregnancy.