Background and aim: Ischemia/reperfusion (I/R) injury of the liver is an important clinical problem encountered in hepatic failure after shock, liver transplantation, or liver surgery, and in diverse situations, including heart failure, liver trauma, and blood occlusion to the liver. Liver functions are decreased in old age and oxygen radicals probably mediate some of the structural and functional alterations associated with ageing and with reperfusion of ischemic liver. This study aimed at investigating the effects of pre-treatment with the antioxidant selenium in an experimental model of hepatic I/R in old female rats. Materials and Methods: The study was carried out on 40 old female Wistar albino rats(250-300 grams), allocated into 4 groups; I. Sham-operated control group, II. Partial liver I/R group, under anaesthesia with pentobarbital 40 mg/kg B.W. subjected for partial hepatic ischemia for 20 minutes followed by reperfusion for 20 minutes, III. Selenium pretreated group with 20 µg/Kg B.W. i.p. for 2 weeks followed by partial liver I/R, and IV. Selenium treated group for 2 weeks. At the end of experimental procedure, blood samples were collected, and the separated plasma was used for determination of liver enzymes (SGPT, SGOT & γ-GT) and plasma total antioxidant capacity (TAC). Liver malondialdehyde (MDA) was measured and liver tissue was examined histologically. Results: Values are in Mean ± SEM, compared by ANOVA. Compared to the sham operated control, in partial liver I/R group; significant increase in SGPT (373.8±35.5 vs 40±5.1, IU/L) SGOT(364.9±7.1 vs180.9±18.8, IU/L), γ-GT (11.7±1.9 vs 6.5±0.87, IU/L) and liver malondialdehyde (24.9±1.2 vs 17.2±1.5, µM/gm wet tissue) and a significant decrease in total antioxidant capacity (0.35±0.05 vs 0.66±0.07, mM/L), associated with leukocyte infiltration of liver tissue. Although selenium treatment for 2 weeks had a non-significant improving effect on normal liver functions of old rats (SGPT: 21.1±3.7, SGOT: 173.1±11.2, γ-GT:6.9±1.2, MDA: 18.4±2.8, TAC: 0.68±0.04), selenium pretreatment for 2 weeks before partial liver I/R resulted in amelioration of the I/R hepatic injury, evidenced by the significant decrease in liver enzymes SGPT (286.3±20.9), SGOT (312.1±8.9), γ-GT (5.5±0.54) and liver MDA (19.7±1.1) and a significant increase in plasma TAC (0.72±0.05) associated with decreased leukocyte infiltration of liver tissue when compared to the partial liver I/R group. Conclusion: Liver I/R in aged rats induces oxidative stress that markedly impairs liver functions and structure. Pretreatment with the dietary antioxidant selenium slightly improves liver functions in old rats, and of much value in protection of the liver of aged rats against I/R injury.