The inflammatory response in large vessels involves the up-regulation of key vascular adhesion molecules, such as intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1, by pro-inflammatory cytokines in the development of atherosclerosis. Chlorella has been shown to possess strong anti-inflammatory effect and to suppress high fat diet-induced atherosclerosis. The aim of this study is to investigate the possible preventing role of Sporopollenin derived from Chlorella against pro-inflammatory cytokine-induced expression of vascular adhesion molecules and endothelial permeability. Endothelial SVEC cells were treated with conditioned media (normal culture media contains 50% of LPS-activated macrophage culture media, in which contained IL-6, TNF-α and MCP-1, with and without Sporopollenin (0.5 and 1 mg/mL). Indomethacin (0.25 mM) was used as a positive control. Productions and mRNA expression of ICAM-1 and VCAM-1 and endothelial permeability were monitored. ICAM-1 and VCAM-1 production and gene expression were all induced by the conditioned media. In the concentration ranges that were devoid of cytotoxicty, the inductions of ICAM-1 and VCAM-1 production and mRNA expression by conditioned media were significantly suppressed by Sporopollenin. Indomethacin was only effectively in preventing the conditioned culture media-induced VCAM-1 production. Cell permeability was also increased in the presence of conditioned culture media. The increased permeability was also inhibited by Sporopollenin but not by indomethacin. These data indicate that Sporopollenin can be a potential material in preventing chronic inflammatory-related vascular diseases.