The exciting discovery of the Greenland sharks (Somniosus microcephalus) life span, which is at least 272 years and may extend to 500 years, is of particular interest with regards to the cardiovascular system and age associated diseases such as fibrosis [1]. Age-related fibrosis is currently accepted as an unavoidable process characterised by harmful degenerative changes that result in collagen deposition within the extracellular matrix [2]. In this study we investigated the epicardium, compacta, transition zone and spongiosum of Picrosirius red stained tissue sections of seven Greenland shark ventricles that ranged from 150 to 250 years old. We used data from brightfield and cross-polarised light microscopy to quantitatively analyse these stained sections using Image J. We report significant collagen deposition in all layers of the ventricle with the epicardium especially, demonstrating extensive (45.97±2.96 %) fibrosis across all specimens. Variations in collagen deposition showed a positive correlation with the age of the sharks as expected. The coherency of the collagen fibres in all four layers of the ventricle across all specimens varied significantly, with the spongiosum being the least ordered and the epicardium being the most. The extent of fibrosis in the Greenland shark may impact its ability to fill with blood and volume-regulate cardiac output [3]. This is the first quantitative analysis of fibrosis in the heart of the world’s oldest vertebrate and it allows rare insight into collagen deposition at varying extremes of age.