Puerto de la Cruz, Tenerife (2003) J Physiol 548P,
P1
Poster Communications: Random insertions of GFP analogues into the hslo channel
Teresa Giraldez*, Douglas L. Sheridan†, Thomas E. Hughes‡ and Frederick J. Sigworth*
*Department of Cellular & Molecular Physiology, Yale University Medical School, New Haven, CT 06520, †Interdepartamental Neuroscience Program, Yale University Medical School, New Haven, CT 06520 and ‡ Department of Ophthalmology & Visual Science, Yale University Medical School, New Haven, CT 06520, USA
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In the past, analogues of green fluorescent protein (GFP) have been inserted randomly into several proteins using the Tn5 transposon (Sheridan et al. 2002). We have used this technique to randomly insert cyan fluorescent protein (CFP) or yellow fluorescent protein (YFP) sequence into multiple sites of the large conductance voltage- and calcium-dependent hslo channel (see Fig. 1). We have generated a library of 56 hslo fusion proteins. Coming next will be tests for function of these constructs.
We thank Kate Klemic for helpful suggestions, and Klara Jacobsdottir for technical support.
Where applicable, experiments conform with Society ethical requirements.
